| 基因重组细胞在环境样品多氯联苯检测中的应用 |
| 摘要点击 1944 全文点击 2739 投稿时间：2003-03-06 修订日期：2003-05-28 |
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| 中文关键词 基因重组细胞 多氯联苯 环境样品 |
| 英文关键词 gene-recombinated cell PCBs compounds environmental sample |
| 作者 | 单位 | | 徐挺 | 南京大学环境学院,南京,210093 | | 孔繁翔 | 南京大学环境学院,南京,210093 | | 孙成 | 南京大学环境学院,南京,210093 | | 谈健康 | 南京大学环境学院,南京,210093 | | 韩小波 | 南京大学环境学院,南京,210093 | | 阎荣 | 南京大学环境学院,南京,210093 |
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| 中文摘要 |
| 为发展快速、简便和廉价的检测环境和生物样品中的多氯联苯技术,本研究利用重组有绿色荧光蛋白(GFP)和荧光素酶(Luc)报告基因的2个细胞系,检测从野外环境中所采集的水、底泥和生物样品中的多氯联苯的含量.研究结果表明,GFP和Luc荧光强度与多氯联苯标样浓度的相关性很好,相关系数分别达到0.99188和0.98239;具有很好的剂量-效应关系.与气相色谱-电子捕获器法(GC-ECD)的仪器分析比较,GFP和Luc的荧光强度与环境样品中的多氯联苯化合物含量也具有很好的相关性.因此可用于受多氯联苯污染的环境样品筛选和半定量快速、简便、廉价检测.
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| 英文摘要 |
| To develop novel and rapid bioassay systems for detection of PCBs in environmental and biological samples, two lines of gene-recombinated cells containing reporter gene, green fluorescent protein (GFP) and luciferase (Luc), were be chosen to detect the PCBs in the water, sediment and biological samples from environment. The result showed that the correlation between RFU of GFP and Luc activity and PCBs standard sample concentration was fine, r was 0.99188 and 0.98239 respectively. Compared with the instrument analysis with GC-ECD, the correlation between RFU of GFP and Luc activity and PCBs concentration in environmental and biological extracts was also fine. The method offered a new way of screening and semi-quantitative bioassay for PCBs compounds in environmental and biological samples. |
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