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多氯代二苯并-对-二噁的微生物降解
摘要点击 1125  全文点击 1433  投稿时间:2000-07-14  
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中文关键词  多氯代二苯并对二噁(PCDDs)  微生物降解  生物积累  降解菌筛选  菌种鉴定
英文关键词  polychlorinated dibenzo-p-dioxins(PCDDs), microbial degradation, bioaccumulation, bacterial isolation, identification of bacterial strains
作者单位
杜秀英 中国科学院生态环境研究中心,北京100085 
竺乃恺 中国科学院生态环境研究中心,北京100085 
夏希娟 中国科学院生态环境研究中心,北京100085 
包志诚 中国科学院生态环境研究中心,北京100085 
徐晓白 中国科学院生态环境研究中心,北京100085 
中文摘要
      从多氯代二苯并-对-二噁(PCDDs)污染的土壤和含氧沉积物中分离筛选出8株降解PCDDs的菌株,均能以一氯代和二氯代二为单一碳源和能源生长并使其降解,多数几乎不能降解三氯代二噁.但是,用邻二氯苯作为初级营养共代谢物,可以增强菌株对较高氯代二噁(如三氯代和四氯代二噁)的降解能力.利用所筛选菌株中的1株,经鉴定为假单胞菌EE41(Pseudomonas sp.EE41).降解试验结果表明,1,2,3-TrCDD在浓度为1.2mg/L时3周内可降解33%,2,3,7,8-TCDD在0.1 mg/L时3周内最多可降解37.8%.试验的高氯代二噁(pCDD,H6-CDD,H7-CDD和OCDD)则只被菌体强烈吸收并积累,却不能被降解.
英文摘要
      PCDDs-degrading bacterial strains were isolated from soil and oxic-sediments polluted by PCDDs. One of them was identified as Pseudomonas sp. EE41. They are capable of growing on and degrade mono- and di-chlorinated dibenzo-p-dioxins as a sole carbon source. The biodegradability of TrCDD and TCDD can be enhanced through primary nutrient of co-metabolism of o-CDB, thus, 1,2,3-TrCDD and 2,3,7,8-TCDD were degraded by 33% (at the concentration of 1.2mg/L, within 3 weeks) and 37.8 % (at the concentration of 0.1mg/L, within 3 weeks) respectively. Most highly chlorinated dibenzo-p-dioxins (P-CDD, H6-, H7-CDD and OCDD) tested in this study were accumulated in bacterial cells, but could not be degraded.

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