| 辣根过氧化物酶处理酚和氯酚的催化特性研究 |
| 摘要点击 816 全文点击 2848 投稿时间：1997-06-16 |
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| 中文关键词 辣根过氧化物酶 苯酚 氯酚 废水处理 造纸废水 酶催化反应 动力学参数 |
| 英文关键词 horseradish peroxidase phenol chlorophenol wastewater treatment pulp-paper wastewater catalysis reaction kinetic parameters |
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| 中文摘要 |
| 为了研究辣根过氧化物酶处理酚和氯酚的动力学过程,采用人工配水试验,测定了pH对酶催化反应的影响、H2O2抑制酶催化反应的动力学参数和不同底物的酶催化反应动力学参数。结果表明,在试验的pH范围内,pH为9时过氧化物酶对苯酚和对氯苯酚的去除效率最高,分别达到99.5%和95%.在较低浓度时污染物去除效率随H2O2浓度的升高而增加,较高浓度H2O2对酶催化反应有一定的抑制效应,但这种抑制作用对酶促反应的影响不大。对氯苯酚、苯酚和间氯苯酚的酶催化反应米氏常数Km依次增加,对酶的亲和性逐渐降低,间氯苯酚最难去除。根据酶反应动力学特征判定,辣根过氧化物酶催化酚形成自由基的氧化反应属于乒乓双双反应机制。 |
| 英文摘要 |
| The removal of phenol and chlorophenol from waste water catalyzed by horseradish peroxidase was studied in this paper to understand the kinetic features of this reaction.The effects of pH,H2O2 concentration and substrate type on the removal of pollutants were discussed in detail.The results showed that the optimum pH for the removal efficiency of phenol and chlorophenol was 9.0 in the test pH range of 5.0 to 9.0.The results also indicated that there was an optimum H2O2 concentration for pollutants removal catalyzed by horseradish peroxidase and H2O2 at concentrations higher than the optimum value had inhibition effect on pollutant removal.But the inhibition effect of higher H2O2 concentrations was very small and had little influence on the reaction.Several substrates were compared on their affinity with horseradish peroxidase according to the kinetics parameters of reactions.The results showed that phenol and 4 chlorophenol were more easy to be removed than 3-chlorophenol.It was also concluded in this paper that oxidation reaction catalyzed by horseradish peroxidase had the characteristic of Ping-Pang reaction. |
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