| 有机磷农药对不同生物来源的胆碱酯酶选择性抑制的研究 |
| 摘要点击 744 全文点击 1797 投稿时间：1995-11-20 |
| 查看HTML全文
查看全文 查看/发表评论 下载PDF阅读器 |
| 中文关键词 有机磷农药 胆碱酯酶 选择性抑制 酶电极 |
| 英文关键词 organophosphorus insecticides acetyl cholinesterases selective inhibition enzyme electrode |
|
| 中文摘要 |
| 用酶电极法研究了有机磷农药对3种不同生物来源的乙酰胆碱酯酶(AchE)的选择性抑制,发现敌敌畏对家蝇的AchE抑制最强,电鳐次之,鸡肝最弱。同时研究了5种农药对电鳐AchE的选择性抑制能力,发现9.8×10-8mol/L的辛硫磷即可抑制其活性的10%.给出了各种农药的检出限。实验还证明在20-45℃内温度对固定化AchE活性的影响不大,讨论了影响其活性的最佳pH值,离子强度和产生选择性抑制的机制。 |
| 英文摘要 |
| The enzyme electrode method was used to study the selective inhibition of acetyl cholinesterases(AchE)extracted from three different biosources by organophosphorus insecticides.It was found that the inhibition extent of AchE by DDVP follows the order of fly,electric fish, chicken liver.In addition the capacity of five kinds of insecticides to selective inhibition AchE extracted from electric fish was studied.It was found that 9.8×10-8 mol/L phoxim is sufficient enough to inhibit 10% of AchE's mobility of electric fish.The detection limits of various kind of insecticides are also presented in this paper.The experiment also showed that in the range of 20-45℃ temperature would exert almost no influence on the mobility of AchE.The optimum pH value,ionic strength and the mechanism of selective inhibition which affect the mobility of AChE were discussed. |
