| 固定化酶催化氧化去除水溶液中酚的研究 |
| 摘要点击 729 全文点击 1064 投稿时间：1995-10-22 |
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| 中文关键词 固定化酶 酶催化氧化 辣根广氧化物酶 酚 |
| 英文关键词 immobilized enzyme enzyme catalyzed oxidation horseradish peroxidase phenol |
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| 中文摘要 |
| 用海藻酸钙凝胶包埋辣根过氧比物酶制成固定化酶,以过氧化氢为氧化剂,进行固定化酶催化氧化去除水溶液中酚的实验研究。对于100ml酚含量为100mg/L的单一苯酚或邻氯基酚水溶液,控制条件为:3%海藻酸钠液30ml,凝胶粒径1mm,固定比时间1h,酶包埋量50u,反应时间1h,pH6及过氧化氢加入量2mmol,单一苯酚和单一邻氨基酚水溶液的酚去除率可分别超过80%和70%.对混合酚水溶液的处理,存在明显的协同效应。制备的固定化酶具有一定的重复使用性能。 |
| 英文摘要 |
| The removal of phenols from aqueous solution by immobilized enzyme catalytic oxidation was studied.The immobilized enzyme was prepared by containing horseradish peroxidase with calcium alginate gel,and hydrogen peroxide was used as oxidizing agent. For 100 ml phenol or oaminophenol aqueous solution containing 100 mg/L phenol, under the following appropriate conditions:3% sodium alginate solution 30 ml,gel particle diameter 1 mm, immobilization time 1h,containing amount of enzyme 50 u,reaction time 1h, pH 6 and hydrogen peroxide dose 2 mmol,the removal rates of phenol and oaminophenol exceeded 80% and 70% respectively.There was synergistic effect between various phenols in the treatment of mixed phenol aqueous solution.The treatment result after mixing different phenol wastewaters is better than their separate treatment result. The immobilized enzyme prepared has fair repeating usage performance. |
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