| 光合细菌与酵母跨界融合子降解味精废水性能测定 |
| 摘要点击 1740 全文点击 1864 投稿时间：1995-09-26 |
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| 中文关键词 球形红假单胞菌 酿酒酵母 原生质体融合 味精废水 动力学参数 |
| 英文关键词 Rh.sphaeroides S.cerevisiae fusant monosodium glutamate wastewater biodegradation kinetic |
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| 中文摘要 |
| 为了筛选出能综合双亲优势的杂种细胞,为废水资源化生产SCP提供更为理想的菌株来源,将原核球形红假单胞菌细胞(NtrSms)与真核酿酒酵母细胞(NtsSmr)的原生质体融合,构建成跨界杂种细胞(NtrSmr).从中筛选出F13,F15,F20三株高絮凝性融合细胞,在起始BOD5浓度为191-690mg/L.pH7.0.30℃的味精废水中振荡反应7-8h,测得融合细胞和双亲菌株降解利用有机污染物的系列反应动力学参数μmax,qmax,Kaμ,KsqY和Kd.发现融合细胞降解废水的性能具有优势,利于废水资源化处理。 |
| 英文摘要 |
| The protoplasts of Rhodopseudomonas sphaerndes(eukaryote,NtrSms)and Saccharomyces cerevisiae (prokaryote, NtsSmr)were induced with PEG(MW=6000)to fuse for the construction of the fusant cell (NtrSmr).The fusants F13,F15 and F20 screened from the'fusion production with high flocculation were used to test the kinetics parameters for degradation of MSG wastewater in the shaking reaction for 7-8 h,while the initial BOD5 concentration of the wastewater were from 191 to 690 mg/L.The values of maximum specific growth rate μmax. and its half velocity constant Ksμ,the maximum specific degradation rate qmaxand its half velocity constant Ksq the true growth yield coefficient Y and the endogenous decay rate coefficient Kd were measured in the reaction.The results of this research suggest that the fusants F13,F15 and F20 have better ability for degradation.of the organic waste in MSG wastewater. |
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