| 导数同步荧光法测定硒 |
| 摘要点击 1438 全文点击 1531 投稿时间：1994-12-05 |
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| 中文关键词 硒 导数同步荧光 2 3－二氨基萘 |
| 英文关键词 selenium synchronous spectrofluorimetry derivative spectrofluorimetry |
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| 中文摘要 |
| 对硒与2,3-二氨基萘(DAN)络合物在有机溶剂中的同步荧光光谱进行了研究。当同步荧光光谱的Δλ选择140nm时,可获得-锐形单峰光谱,在此基础上获得了一阶导数同步荧光和二阶导数同步荧光的最佳实验条件。用一阶导数同步荧光定量测定硒可提高方法的灵敏度和改善精密度,在仪器的中挡灵敏范围,硒的检测限为1.5ng,硒浓度在0—250ng时,硒浓度与荧光强度的直线关系是y=0.44x—0.18,相关系数(r)0.9985,10次测定的变异系数(CV%)为6.7%。对水样、血液和头发中的硒进行了实测。 |
| 英文摘要 |
| The synchronous fluorescence spectra of fluorescent chelate of selenium with 2,3-diaminophthalene(DAN) and its synchronous scanning derivative spectra were studied. The specific Δλof the synchronous spectra was found to be 140 urn. The first derivative spectrofluorimetry for the determination of selenium with DAN was described. The limits of detection were 1.5, 1.9 and 3.0 for the first derivative,second derivative and normal spectrofluorimetric technique,respectively. Coefficient of variation was 6.7 for the first derivative spectrofluorimetry,and 9.2 for the normal spectrofluorimetry. |
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