| 单克隆抗体酶免疫分析法测定大米中杀虫脒残留量 |
| 摘要点击 926 全文点击 901 投稿时间：1995-03-08 |
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| 中文关键词 杀虫脒 农药残留量 免疫分析法 |
| 英文关键词 chlordimeform pesticide residue analysis enzyme-linked immunosorbent assay (ELISA) |
| 作者 | 单位 | | 余万俊 | 华西医科大学公共卫生学院, 基础医学院, 成都 610041 | | 鲁长豪 | 华西医科大学公共卫生学院, 基础医学院, 成都 610041 | | 肖志芳 | 华西医科大学公共卫生学院, 基础医学院, 成都 610041 | | 牟家婉 | 华西医科大学公共卫生学院, 基础医学院, 成都 610041 | | 蒋中华 | 华西医科大学公共卫生学院, 基础医学院, 成都 610041 |
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| 中文摘要 |
| 用抗杀虫脒单克隆抗体为试剂,建立了间接竞争法、直接竞争法和标记抗原的竞争法等3种测定大米中杀虫眯残留量的ELISA法,标准曲线上抑制率为50%时对应的杀虫眯浓度分别为1.3、1.7和5.3ng/ml.大米样品用甲醇提取,提取液经吸附剂柱简单净化后即可用上述ELISA对痕量杀虫眯进行定量。使用直接竞争法时,样品加标回收率为90%—116%,平行测定的相对标准偏差为5.l%—16%,方法检出限为0.5ng/g。笔者还对孵育介质pH和温度、提取剂及样品基体等因素对定量曲线的影响进行了研究和讨论。 |
| 英文摘要 |
| Three sensitive enzyme-linked immunosorbent assays(ELISAs) indirect competitive ELISA,direct competitive ELISA,and reverse direct competitive ELISA-with immobilized antibody were developed for the determination of chlordimeform residues in rice. Their IC50 are of 1.3,1.7 and 5.3 ng/ml,respectively. Samples were homogenized with methanol in a blender or in an ultrasonic disintegrator. The extracts could be measured directly after dilution,or underwent a simple clean up procedure,which could improve the accuracy of the ELISAs. Effects of PH in the reaction medium,the incubation temperature,the extracting agent and the sample matrix on the detection sensitivities were also studied and discussed. |
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