| HPLC法研究DNA及其甲基修饰物的酸性介质降解 |
| 摘要点击 769 全文点击 947 投稿时间：1994-10-20 |
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| 中文关键词 高效液相色谱 DNA 亚硝基脲 降解 |
| 英文关键词 HPLC DNA MNU adduct |
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| 中文摘要 |
| 用HPLC梯度洗脱法研究了DNA在盐酸、高氨酸、三氯乙酸、三氟乙酸以及甲酸等酸性介质中的降解碱基的分离和确定。给出了MNU与DNA的加合产物的降解产率。对流动相组成、流率、柱温、梯度速率、pH值、盐浓度以及缓冲液浓度对容量因子(K')的影响分别进行了讨论。结果表明,DNA在盐酸介质中可以得到最好的碱基回收率,MNU与DNA的加合产物在1mol/L盐酸介质中、20min条件下,可获得较好的碱基回收效果。 |
| 英文摘要 |
| he determination and separation of bases from the degradation products of DNA in hydrochloric acid,perchloric acid,trichloroacetic acid,trifluoroacetic acid and formic acid were described. The hydrolysis yields of DNA adduct products of MNU also were presented. The effects of mobile phase composition,flow rate,column temperature,gradient ramp,PH value,salt concentration,and buffer concentration on capacity factors(K') were respectively discussed. The experimental results showed that hydrochloric acid is a .favorable hydrolytical medium for DNA degradation and high recovery of DNA bases can be obtained. The optimum hydrolytical conditions for adduct products of DNA of MNU were 1 mol/L hydrochloric acid,20 min,and 80—100℃. |
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