| 厌氧污泥胞外多聚物的提取、测定法选择 |
| 摘要点击 1198 全文点击 1950 修订日期：1993-06-19 |
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| 中文关键词 厌氧污泥 胞外多聚物 提取法 硫酸提取法 |
| 英文关键词 anaerobic sludge extracellular polymer extraction method sulfuric acid process for ECP extraction |
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| 中文摘要 |
| 选用6种方法,对4种不同性状的厌氧污泥的胞外多聚物(ECP)进行提取试验。此外,采用2种方法测定提取液中多糖浓度,还测定核酸浓度以检查细胞的破坏程度。通过对比,认为硫酸法提取的ECP较完全,对细胞的破坏作用小,而苯酚-硫酸法较适于测定提取液中的多糖含量。文中还对厌氧污泥ECP的含量、成分作了分析和探讨。 |
| 英文摘要 |
| Extracellular polymers(ECPs)were extracted from 4 kinds of anaerobic sludge by using 6 different ECPs extracting processes,i.e.,sulfuric acid process, sodium hydroxide process,ethylenediaminetetraacetie acid (EDTA) process, boiling benzene process,distilled water process and steaming process,which have been used to extract ECPs from activated sludge.The concentrations of polysaccharides in the extracts were determined by both the phenol-sulfuric acid method and the anthrone-sulfuric acid method, and the concentrations of nucleic acid were also measured to examine the degree of cells damaged. The results show that the sulfuric acid process can extract more ECPs from anaerobic sludges, with a less degree of damage to cells, than other processes. The phenol-sulfuric acid method was found more suitable to measure the concentration of polysaccharide.The level and composition Of ECPs in anaerobic sludges were also discussed. |
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