铬革渣资源化处理研究Ⅸ──胶原蛋白分子量及分子量分布 |
摘要点击 890 全文点击 943 修订日期:1993-07-30 |
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中文关键词 铬革渣 分子量 凝胶色谱 |
英文关键词 chrome leather scrap molecularweight gel chromatography |
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中文摘要 |
以含0.02%叠氮钠的蒸馏水为流动相,宽分布T10和T70葡聚糖为标样,用水相凝胶色谱法(WPGC)测定了从铬革渣中按不同条件提取的胶原蛋白的分子量及分子量分布。在实验中设定的反应时间和介质的pH值范围内胶原蛋白的数均分子量(Mn)均低于4000,分子量分布较宽(d=Mw/Mn=3.19-8.35)。 |
英文摘要 |
The moleculat weight and its distribution of the collagen protein were determined with a water-phase gel chromatography (WPGC). The collagen protein was seperated from chrome leather scraps under different reaction conditions. In WPGC, distilled water was used as solvent,containing 0. 02%NaN2,and dextran with a broad molecular weight distribution as standard. In the designed reaction time and PH value range, the number average molecular weight (Mn) of collagen Protein Was below 4000,and the molecular weight distribution proves to be wide (d=Mw/Mn=3.19-8. 35). |