用铬革渣制备L-脯氨酸等六种氨基酸 |
摘要点击 1766 全文点击 1821 投稿时间:1988-09-05 |
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英文关键词 |
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中文摘要 |
铬革渣经碱性处理提取蛋白质,用6mol HCl水解,活性炭脱色,调节pH,由732#阳离子交换树脂初步分离,得到的脯氨酸组分再由717#阴离子交换树脂分离,所得L-脯氨酸洗脱液减压蒸发浓缩,用无水乙醇结晶,得色谱纯L-脯氨酸。在732#阳离子交换树脂上最后流出的是精氨酸,经处理得色谱纯L-精氨酸盐酸盐。在717#阴离子交换树脂上分离可分别获得L-谷氨酸、L-天门冬氨酸、L-丙氨酸和甘氨酸。 |
英文摘要 |
The scraps of chrome leather is a tanning waste. The process of reclaiming six amino-acids was operated as follows: de-chroming of the scraps was adopted by basic hydrolysis with calcium oxide, and the protein extracted; the protein was hydrolyzed with 6 mol HC1; the hydroly-sate was declorized with activated carbon; then separated by 732 cation exchange resin (H form) and 717 anion exchange resin (OH form) respectively. L-Arg, L-Pro, L-Asp, L-Ala and Gly were obtained. |
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