| 青藏高原内陆湖泊氮转化微生物群落结构的季节性响应特征 |
| 摘要点击 448 全文点击 10 投稿时间：2025-07-28 修订日期：2025-10-14 |
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| 中文关键词 高原内陆湖泊 微生物 氮转化 功能基因 群落构建 青藏高原（QXP） |
| 英文关键词 plateau inland lake microbe nitrogen transformation functional genes assembly processes Qinghai-Xizang Plateau（QXP） |
| DOI 10.13227/j.hjkx.202507365 |
| 作者 | 单位 | E-mail | | 王丹丹 | 青海大学土木水利学院, 黄河上游生态保护与高质量发展实验室, 水利部江河源区水生态治理与保护重点实验室, 西宁 810016 | 2023990002@qhu.edu.cn | | 谢燕琴 | 青海大学土木水利学院, 黄河上游生态保护与高质量发展实验室, 水利部江河源区水生态治理与保护重点实验室, 西宁 810016 | | | 黄跃飞 | 青海大学土木水利学院, 黄河上游生态保护与高质量发展实验室, 水利部江河源区水生态治理与保护重点实验室, 西宁 810016 | yuefeihuang@tsinghua.edu.cn | | 贾海超 | 青海大学土木水利学院, 黄河上游生态保护与高质量发展实验室, 水利部江河源区水生态治理与保护重点实验室, 西宁 810016 | |
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| 中文摘要 |
| 研究青藏高原内陆湖泊微生物菌群氮转化特征对深入理解区域湖泊生态系统氮元素收支平衡和生物地球化学循环具有重要意义. 基于宏基因测序技术构建氮转化微生物非冗余基因库和多元统计分析，对青藏高原内陆湖泊氮转化微生物群落特征、驱动因素和构建机制进行解析. 结果表明：①细菌是高原内陆湖泊水体氮转化微生物的主要类群，夏季和冬季参与氮转化过程的优势细菌门均为假单胞菌门（Pseudomonadota），不同季节不同样点的平均占比分别为39.35%和35.09%. 夏季和冬季的优势细菌属为Candidatus_planktophila（不同样点的平均占比为4.04%）和unclassified_c__Actinomycetes（不同样点的平均占比为7.91%）. ②影响不同季节氮转化微生物群落结构最显著的环境因子是盐度和溶解氧. ③不同季节湖泊水体氮转化微生物功能基因丰度存在差异，反硝化过程是高原内陆湖泊不同季节水体微生物参与最为广泛的过程. 驱动不同季节氮转化基因丰度的环境因子是海拔、水温、总溶解固体和盐度. 整体上看，内陆湖氮转化微生物群落构建过程存在明显的时空异质性. 相关结果可为高原内陆湖泊生态系统氮转化微生物的理解提供数据支撑，同时对湖泊水生态治理和区域温室气体的排放提供理论依据. |
| 英文摘要 |
| Studying the nitrogen transformation characteristics of microbial communities in the inland lakes of the Qinghai-Xizang Plateau （QXP） is of great significance for a deeper understanding of the nitrogen budget balance and biogeochemical cycling in the regional lake ecosystems. Based on metagenomic sequencing technology， this study constructed a non-redundant gene library of nitrogen-transforming microorganisms and conducted multivariate statistical analysis to explore the characteristics and assembly mechanisms of nitrogen-transforming microbial communities in inland lakes on the QXP. The results indicate： ① Bacteria were the main group of nitrogen transformation microbes in the plateau inland lakes. The dominant bacterial phylum involved in nitrogen conversion both in summer and winter was Pseudomonadota， with an average proportion of 39.35% and 35.09% at different sampling sites in different seasons， respectively. The dominant bacterial genera in summer and winter were Candida_planktophila （the average proportion of different sampling sites was 4.04%） and unclassified_c_Actinomycetes （the average proportion of different sampling sites was 7.91%）. ② Salinity and dissolved oxygen were the most significant environmental factors affecting the microbial community structure of nitrogen transformation in different seasons. There were differences in the process of nitrogen transformation microbial community assembly in different seasons and different sampling lakes. ③ There were differences in the abundance of functional genes of nitrogen transformation microbes in different seasons， and denitrification was the most widely involved process of microbial communities in the plateau inland lakes in different seasons. The environmental factors driving the abundance of nitrogen transformation genes in different seasons were altitude， water temperature， total dissolved solids， and salinity. Overall， there was significant spatiotemporal heterogeneity in the assembly process of nitrogen transformation microbial communities in the plateau inland lakes. The results of this study can provide data support for the understanding of nitrogen-transforming microbes in the plateau inland lake ecosystem and provide a theoretical basis for lake water ecological management and regional greenhouse gas emissions. |
