| 中国西南喀斯特地区铅锌矿尾砂库植被恢复对土壤微生物群落结构影响 |
| 摘要点击 2525 全文点击 483 投稿时间：2024-09-11 修订日期：2024-11-29 |
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| 中文关键词 尾矿 植被恢复 微生物 群落分布 影响因素 |
| 英文关键词 tailings vegetation restoration microorganisms community distribution influencing factors |
| DOI 10.13227/j.hjkx.202409142 |
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| 中文摘要 |
| 为探究中国西南喀斯特地区铅锌矿尾砂库在植被恢复过程中土壤微生物群落变化特征及影响因素，以广西阳朔铅锌矿尾砂库修复区为研究对象，采用空间代替时间的方法，以不同恢复区域代表不同恢复阶段. 采用高通量测序技术以及相生物信息学分析技术对尾砂库不同恢复区域的土壤理化性质及微生物群落结构进行分析，同时探究微生物群落与环境因子之间的潜在联系，寻找关键驱动因子. 结果表明：①不同恢复区域的土壤MC、pH、SOM、Zn和Cu存在显著差异；②门水平上，变形菌门（Proteobacteria）为主要优势物种，属水平上鞘氨醇单胞菌属（Sphingomonas）为主要优势物种. 人为恢复区微生物丰度与多样性最高，自然过渡区微生物种群与人为恢复区及对照区存在显著差异，人为恢复区与自然过渡区微生物群落构建以确定性为主，对照区以随机性为主；③RDA分析结果表明Cu是影响微生物群落分布的关键环境因子，与变形菌门（Proteobacteria）和Patescibacteria门显著相关. pH、TN和SOM对微生物种群影响较为显著，TN与酸杆菌门（Acidobacteria）显著相关. 研究结果对日后进行矿区生态恢复或者植被重建具有理论指导意义. |
| 英文摘要 |
| To investigate the characteristics and influencing factors of soil microbial community changes during vegetation restoration of lead-zinc mine tailing ponds in karst areas of southwestern China， the restoration area of lead-zinc mine tailing ponds in Yangshuo was taken as the research object， and the spatial （instead of the temporal） approach was adopted， with different restoration areas representing different restoration stages. High-throughput sequencing technology and phase bioinformatics analysis were used to analyze the soil physicochemical properties and microbial community structure in the different restoration areas of the tailing ponds and， at the same time， to explore the potential connection between microbial communities and environmental factors and search for key driving factors. The study obtained the following results： ① Significant differences in soil water content， pH， and SOM existed among different restoration areas. ② Proteobacteria was the dominant group at the phylum level， and Sphingomonas was the dominant genera. Microbial abundance and diversity were highest in the anthropogenic restoration area. Moreover， the microbial populations in the natural transition area differed significantly from those in the anthropogenic restoration area and the control area. The microbial communities in the anthropogenic restoration area and the natural transition area were constructed with determinism， whereas those of the control area were random. ③ The RDA analysis results showed that Cu was the key environmental factor affecting the distribution of microbial communities and was significantly associated with Proteobacteria and Patescibacteria. pH， TN， and SOM had a more significant effect on microbial populations， and TN was significantly associated with Acidobacteria. The conclusions of this study provide theoretical guidance for future ecological restoration or revegetation of mining areas. |
