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芦芽山华北落叶松林土壤剖面细菌群落分布格局
摘要点击 938  全文点击 330  投稿时间:2022-06-19  修订日期:2022-08-22
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中文关键词  华北落叶松  土壤剖面  细菌群落  结构与多样性  分布格局
英文关键词  Larix principis-rupprechtii  soil profile  bacterial community  structure and diversity  distribution pattern
作者单位E-mail
毛晓雅 山西大学黄土高原研究所, 黄土高原生态恢复山西省重点实验室, 太原 030006 1274802496@qq.com 
刘晋仙 山西大学黄土高原研究所, 黄土高原生态恢复山西省重点实验室, 太原 030006  
贾彤 山西大学黄土高原研究所, 黄土高原生态恢复山西省重点实验室, 太原 030006  
吴铁航 佐治亚州南方大学生物系, 佐治亚州斯泰茨伯勒, 美国  
柴宝峰 山西大学黄土高原研究所, 黄土高原生态恢复山西省重点实验室, 太原 030006 bfchai@sxu.edu.cn 
中文摘要
      微生物群落是维持森林土壤生态系统结构和功能的关键组分.细菌群落在土壤剖面上的垂直分布对森林土壤碳库和土壤营养循环具有重要影响.利用Illumina MiSeq高通量测序技术,分析了芦芽山华北落叶松(Larix principis-rupprechtii)林腐殖质层及0~80 cm土层细菌群落特征,探究影响土壤剖面细菌群落结构的驱动机制.结果表明,细菌群落的α多样性随土壤深度的增加显著单调降低,群落结构在土壤剖面上有显著差异.放线菌门(Actinobacteria)、变形菌门(Proteobacteria)的相对丰度随着土壤深度增加而降低;酸杆菌门(Acidobacteria)和绿弯菌门(Chloroflexi)的相对丰度随土层深度的增加而增加.RDA分析结果表明,土壤NH+4、TC、TS、WCS、pH、NO-3和TP是决定土壤剖面细菌群落结构的重要因子,其中pH的影响最为显著.分子生态网络分析表明,凋落物层和亚表层土壤(10~20 cm)细菌群落的复杂性较高,深层(40~80 cm)土壤细菌群落的复杂性相对较低.变形菌门、酸杆菌门、绿弯菌门和放线菌门在华北落叶松土壤细菌群落结构和稳定性方面发挥重要作用.Tax4Fun的物种功能预测结果显示,沿着土壤剖面深度的增加,微生物的代谢能力逐渐下降.总之,华北落叶松林土壤细菌群落结构沿土壤垂直剖面呈现一定的分布格局,群落复杂性逐渐降低,深层土壤与表层土壤特有细菌类群差异显著.
英文摘要
      Microbial communities are the key component to maintaining the structure and function of forest soil ecosystems. The vertical distribution of bacterial communities on the soil profile has an important impact on forest soil carbon pools and soil nutrient cycling. Using Illumina MiSeq high-throughput sequencing technology, we analyzed the characteristics of bacterial communities in the humus layer and 0-80 cm soil layer of Larix principis-rupprechtii in Luya Mountain, China, to explore the driving mechanisms affecting the structure of bacterial communities in soil profiles. The results showed that the α diversity of bacterial communities decreased significantly with increasing soil depth, and community structure differed significantly across soil profiles. The relative abundance of Actinobacteria and Proteobacteria decreased with increased soil depth, whereas the relative abundance of Acidobacteria and Chloroflexi increased with the increase in soil depth. The results of RDA analysis showed that soil NH+4, TC, TS, WCS, pH, NO-3, and TP were important factors determining the bacterial community structure of the soil profile, among which soil pH had the most significant effect. Molecular ecological network analysis showed that the complexity of bacterial communities in the litter layer and subsurface soil (10-20 cm) was relatively high, whereas the complexity of bacterial communities in deep soil (40-80 cm) was relatively low. Proteobacteria, Acidobacteria, Chloroflexi, and Actinobacteria played important roles in the structure and stability of soil bacterial communities in Larch. The species function prediction of Tax4Fun showed a gradual decline in microbial metabolic capacity along the soil profile. In conclusion, soil bacterial community structure showed a certain distribution pattern along the vertical profile of soil, the community complexity gradually decreased, and the unique bacterial groups of deep soil and surface soil were significantly different.

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