| 西安市景观湖泊水体细菌群落结构分析与代谢功能预测 |
| 摘要点击 2839 全文点击 2573 投稿时间：2022-03-15 修订日期：2022-05-09 |
| 查看HTML全文
查看全文 查看/发表评论 下载PDF阅读器 |
| 中文关键词 城市湖泊 细菌群落 多样性 环境因子 代谢功能 |
| 英文关键词 urban lake bacterial community diversity environmental factors metabolic function |
| 作者 | 单位 | E-mail | | 王敏 | 西安理工大学省部共建西北旱区生态水利国家重点实验室, 西安 710048
西安理工大学水利水电学院, 西安 710048 | wangmin@xaut.edu.cn | | 张雨桐 | 西安理工大学省部共建西北旱区生态水利国家重点实验室, 西安 710048
西安理工大学水利水电学院, 西安 710048 | | | 黄晨 | 西安理工大学省部共建西北旱区生态水利国家重点实验室, 西安 710048
西安理工大学水利水电学院, 西安 710048 | | | 任杰辉 | 西安理工大学省部共建西北旱区生态水利国家重点实验室, 西安 710048
西安理工大学水利水电学院, 西安 710048 | | | 万甜 | 西安理工大学省部共建西北旱区生态水利国家重点实验室, 西安 710048
西安理工大学水利水电学院, 西安 710048 | | | 程文 | 西安理工大学省部共建西北旱区生态水利国家重点实验室, 西安 710048
西安理工大学水利水电学院, 西安 710048 | |
|
| 中文摘要 |
| 湖泊水体中细菌群落多样性及其代谢功能研究对城市景观湖泊水环境健康发展有着重要意义.以汉城湖、南湖、兴庆湖和桃花潭为研究区域，使用Illumina Nova高通量测序技术研究4个不同类型湖泊中群落组成和结构，使用冗余分析方法分析细菌群落与环境因子的相关性，采用Tax4Fun预测细菌群落的代谢功能.结果表明，不同湖泊水体细菌群落多样性各不相同，其中桃花潭α多样性最高，兴庆湖最低，同一湖泊水体的细菌群落结构有聚集趋势；4个湖泊水体的细菌优势门类相似，主要是变形菌门、拟杆菌门和放线菌门，不同之处仅在于细菌的比例；4个湖泊水体属水平细菌群落分布有较大差异；水体理化性质与细菌群落存在显著相关性，其中最关键因子为氮磷营养盐（P<0.05）；Tax4Fun功能预测表明，4个湖泊水体细菌群落代谢功能相似，氨基酸代谢和碳水化合物代谢相对丰度最高，此外，其潜在的耐药性细菌污染较大以及致人类患传染性疾病可能性较大.研究结果有助于了解西安市城市湖泊水环境细菌现状，为城市湖泊治理及可持续发展提供理论依据. |
| 英文摘要 |
| The study of bacterial community diversity and metabolic function in lake water is of great significance to the healthy development of the urban landscape lake water environment. In this study, Hancheng Lake, Nanhu Lake, Xingqing Lake, and Taohuatan Lake were selected as the study area. Illumina Nova high-throughput sequencing technology was used to study the composition and structure of bacterial communities in four different types of lakes. The correlation between bacterial communities and environmental factors was analyzed using the RDA method. Tax4Fun was used to predict the metabolic function of bacterial communities. The results showed that the diversity of bacterial communities was different in different lakes. The α diversity of Taohuatan Lake was the highest, and that of Xingqing Lake was the lowest. The bacterial community structure of the same lake had a trend of aggregation. The dominant phyla of bacteria in the four lakes were similar, mainly Proteobacteria, Bacteroidetes, and Actinobacteria. The difference was only in the proportion of bacteria. Additionally, the distribution of bacterial communities at the genus level in the four lakes was quite different. The physical and chemical properties of lakes were significantly correlated with bacterial community, and the most critical factor was nitrogen and phosphorus nutrients(P<0.05). Tax4Fun function prediction showed that the metabolic function of bacterial communities in the four lakes was similar, and the relative abundance of amino acid metabolism and carbohydrate metabolism was the highest. In addition, the potential drug-resistant bacterial pollution and the possibility of human infectious diseases were higher. These results are helpful for understanding the current situation of bacteria in urban lakes in Xi'an and providing a theoretical basis for the management and sustainable development of urban lakes. |
|
|
|
