| 一体式部分亚硝化-厌氧氨氧化工艺污泥膨胀发生和恢复过程中微生物群落演替及PICRUSt2功能预测分析 |
| 摘要点击 1797 全文点击 486 投稿时间：2021-09-30 修订日期：2021-11-18 |
| 查看HTML全文
查看全文 查看/发表评论 下载PDF阅读器 |
| 中文关键词 厌氧氨氧化(ANAMMOX) 微生物群落 多样性 PICRUSt2 高通量测序 氮代谢 碳代谢 |
| 英文关键词 anaerobic ammonia oxidation (ANAMMOX) microbial community diversity PICRUSt2 high-throughput sequencing nitrogen metabolism carbon metabolism |
| 作者 | 单位 | E-mail | | 李亚男 | 中国科学院生态环境研究中心水污染控制实验室, 北京 100085
中国科学技术大学生命科学学院, 合肥 230026 | yanan08@mail.ustc.edu.cn | | 闫冰 | 中国科学院生态环境研究中心水污染控制实验室, 北京 100085
江西省科学院能源研究所, 南昌 330096 | | | 郑蕊 | 河北省安平县弘嘉环保技术有限公司, 衡水 053600 | | | 姚丽 | 中国科学院生态环境研究中心水污染控制实验室, 北京 100085
中国科学技术大学生命科学学院, 合肥 230026 | | | 隋倩雯 | 中国科学院生态环境研究中心水污染控制实验室, 北京 100085 | | | 魏源送 | 中国科学院生态环境研究中心水污染控制实验室, 北京 100085
中国科学技术大学生命科学学院, 合肥 230026
江西省科学院能源研究所, 南昌 330096 | yswei@rcees.ac.cn |
|
| 中文摘要 |
| 以中试规模的一体式部分亚硝化-厌氧氨氧化（CPNA）工艺为研究对象，通过对其污泥膨胀发生和恢复过程中活性污泥的16S rRNA高通量测序数据进行数据挖掘和分析，结合PISCRUSt2功能预测分析，旨在揭示一体式CPNA工艺污泥膨胀和恢复不同阶段的微生物群落变化及氮、碳代谢特征.结果表明，污泥膨胀和恢复过程中微生物α多样性呈先上升后下降趋势，污泥膨胀阶段氮素转化菌属Nitrosomonas、Candidatus_Brocadia和Thauera相对丰度分别从12.36%、11.86%和0.272%下降至5.97%、8.30%和0.061%，而Candidatus Kuenenia相对丰度保持稳定，丝状菌Levilinea、Longilinea和Turicibacter相对丰度分别从0.031%、0.018%和0.009%上升至0.055%、0.025%和0.033%.PICRUSt2功能预测分析结果表明，总共有47个参与氮代谢的功能酶基因，其中硝化、反硝化、异化性硝酸盐还原（DNRA）、同化硝酸盐还原（ANRA）和固氮反应的功能酶基因相对丰度均发生了变化.污泥膨胀阶段，部分亚硝化功能基因氨单加氧酶基因（pmoABC-amoABC）和羟胺脱氢酶基因hao相对丰度下降，硝酸盐还原功能基因相对丰度膨胀初期升高，随后呈下降趋势.碳代谢分析表明，乙酸钠对CPNA工艺的异养生长具有促进作用，然而乙酸钠的能量代谢和葡萄糖生成并不活跃. |
| 英文摘要 |
| This study was based on the pilot one-stage combined partial nitritation and ANAMMOX process (CPNA), using data mining and analysis of 16S rRNA high-throughput sequencing data of activated sludge in the process of sludge bulking and recovery, combined with PISCRUSt2. The function prediction analysis aimed to reveal the microbial community changes and the characteristics of nitrogen metabolism and carbon metabolism at different stages of sludge bulking and recovery of the one-stage CPNA process. The results of the study showed that the microbial α-diversity in the sludge bulking and recovery process first increased and then declined. The relative abundance of Nitrosomonas, Candidatus_Brocadia, and Thaurea decreased in the sludge-bulking stage from 12.36%, 11.86%, and 0.272% to 5.97%, 8.30%, and 0.061%, whereas the relative abundance of Candidatus Kuenenia remained stable. The relative abundance of Levilinea, Longilinea, and Turicibacter increased from 0.031%, 0.018%, and 0.009% to 0.055%, 0.025%, and 0.033%. The PICRUSt2 function prediction analysis results showed that there were a total of 47 functional enzyme genes involved in nitrogen metabolism, of which nitrification, denitrification, dissimilative nitrate reduction (DNRA), assimilation nitrate reduction (ANRA), and nitrogen fixation were relatively abundant. The degrees of each had changed. During the sludge-bulking stage, the relative abundance of the ammonia monooxygenase gene (pmoABC-amoABC) and the hydroxylamine dehydrogenase gene hao decreased, whereas the relative abundance of the nitrate-reducing gene increased at the initial stage and then showed a downward trend. Carbon metabolism analysis showed that sodium acetate had a promoting effect on the heterotrophic growth of the CPNA process, but the energy metabolism and glucose production of sodium acetate were not active. |
|
|
|
