| 长期储存亚硝化絮状污泥活性的恢复 |
| 摘要点击 2866 全文点击 936 投稿时间：2018-02-22 修订日期：2018-03-13 |
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| 中文关键词 亚硝化絮状污泥 活性恢复 完全混合反应器(CSTR) 微生物菌群结构 |
| 英文关键词 nitrosation flocculent sludge reactivation continuous stirred tank reactor (CSTR) microbial community structure |
| 作者 | 单位 | E-mail | | 李田 | 苏州科技大学环境科学与工程学院, 苏州 215009 | lt690824185@sina.com | | 魏凡凯 | 苏州科技大学环境科学与工程学院, 苏州 215009 | | | 汪裕昌 | 苏州科技大学环境科学与工程学院, 苏州 215009 | | | 沈耀良 | 苏州科技大学环境科学与工程学院, 苏州 215009
江苏省水处理技术与材料协同创新中心, 苏州 215009
江苏省环境科学与工程重点实验室, 苏州 215009 | | | 吴鹏 | 苏州科技大学环境科学与工程学院, 苏州 215009
江苏省水处理技术与材料协同创新中心, 苏州 215009
江苏省环境科学与工程重点实验室, 苏州 215009 | wupengniu@126.com | | 宋吟玲 | 苏州科技大学环境科学与工程学院, 苏州 215009
江苏省水处理技术与材料协同创新中心, 苏州 215009
江苏省环境科学与工程重点实验室, 苏州 215009 | |
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| 中文摘要 |
| 为探究长期储存亚硝化絮状污泥的脱氮性能，采用CSTR反应器，接种4℃下储存了10个月的亚硝化絮状污泥，考察其活性恢复性能，并采用MiSeq高通量测序技术分析了污泥中微生物菌群结构的变化情况.结果表明，控制DO为0.4~0.8 mg·L-1、pH值8左右、温度为（30±1）℃等条件，长期储存亚硝化絮状污泥的活性可以在15 d内迅速恢复，氨氮去除率和亚硝积累率均达到90%以上；此外，污泥颜色由接种初期的灰黑色迅速恢复至棕黄色，SVI值显著降低，MLVSS/MLSS升高，EPS含量明显增加.随着亚硝化性能的恢复，厌氧、发酵微生物被洗脱，Nitrosomonas等氨氧化细菌相对丰度显著增加，同时，Nitrospria等硝化菌的生长得到了有效抑制.经历长期储存的亚硝化絮状污泥可作为实现短程硝化快速启动的接种污泥，更有利于短程硝化工艺的实际应用. |
| 英文摘要 |
| In order to explore the nitrogen removal performance of nitrosation flocculent sludge subject to long-term storage, nitrosation flocculent sludge which had been stored at 4℃ for ten months was inoculated in a continuous stirred tank reactor (CSTR) to investigate its activity recovery performance. MiSeq high throughput sequencing technology was also employed to analyze the structure of the microbial community before and after reactivation. Results showed that, under DO control of DO 0.4-0.8 mg·L-1, pH value of about 8 and temperature of (30±1)℃, the sludge could be reactivated in 15 days. Both the removal efficiency of ammonia nitrogen and the accumulation ratio of nitrite nitrogen were higher than 90%. The color of the sludge quickly changed from grey at early inoculation to brown, while the sludge volume index (SVI) quickly decreased and both mixed liquor volatile suspended solids (MLVSS)/mixed liquor suspended solids (MLSS) and extracellular polymer substances (EPS) significantly increased. With the recovery of nitrosation performance, anaerobic and heterotrophic bacteria were washed out, the relative abundance of ammomia-oxidizing bacteria such as Nitrosomonas significantly increased, while the growth of nitrite-oxidizing bacteria such as Nitrospria was selectively inhibited. Nitrosation flocculent sludge could be inoculated after long-term storage to achieve rapid start-up of partial nitrification and allow practical application of partial nitrification processes. |
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