| 温度对SCSC-S/Fe复合系统脱氮除磷及微生物群落特性的影响 |
| 摘要点击 2937 全文点击 1076 投稿时间：2016-11-01 修订日期：2016-12-19 |
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| 中文关键词 温度 纤维素类降解菌 反硝化细菌 MiSeq高通量测序 微生物群落特性 |
| 英文关键词 temperature cellulose-degrading bacteria denitrifying bacteria MiSeq high throughput sequencing microbial community characteristics |
| 作者 | 单位 | E-mail | | 范军辉 | 北京工业大学建筑工程学院, 北京市水质科学与水环境恢复工程重点实验室, 北京 100124
北京碧水源科技股份有限公司, 北京 100084 | fhjahyq@163.com | | 郝瑞霞 | 北京工业大学建筑工程学院, 北京市水质科学与水环境恢复工程重点实验室, 北京 100124 | haoruixia@bjut.edu.cn | | 朱晓霞 | 北京工业大学建筑工程学院, 北京市水质科学与水环境恢复工程重点实验室, 北京 100124 | | | 万京京 | 北京工业大学建筑工程学院, 北京市水质科学与水环境恢复工程重点实验室, 北京 100124 | | | 刘思远 | 北京工业大学建筑工程学院, 北京市水质科学与水环境恢复工程重点实验室, 北京 100124 | | | 王丽沙 | 北京工业大学建筑工程学院, 北京市水质科学与水环境恢复工程重点实验室, 北京 100124 | |
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| 中文摘要 |
| 为了探究温度对纤维素类降解菌和反硝化细菌的影响，分析了固相纤维素碳源玉米芯+硫铁填料脱氮除磷复合系统（solid carbon source of cellulose and sulfur/sponge iron process，SCSC-S/Fe复合系统）在不同温度下脱氮除磷效果，通过扫描电镜（SEM）和MiSeq高通量测序技术对反应前后玉米芯表面结构及微生物特性进行分析.结果表明，温度从15、20、25℃升高到30℃过程中，该系统TN平均去除率从78.88%增加到92.70%、TP平均去除率从82.58%增加到89.15%；反应后玉米芯表面以球状和杆状微生物为主；纤维素类降解菌所占比例在30℃比20℃时高出11.01%，而反硝化细菌所占比例降低了21.26%.可见，纤维素降解菌比反硝化细菌对温度更敏感，受温度影响更大. |
| 英文摘要 |
| In order to investigate the effect of temperature on the cellulose-degrading bacteria and denitrifying bacteria, the denitrification and phosphorus removal of solid carbon source of cellulose corncob+sulfur/sponge iron nitrogen and phosphorus removal composite system, abbreviated as SCSC-S/Fe, was analyzed under different temperature conditions, and the surface structure and microbial properties of corncob before and after reaction were analyzed by scanning electron microscope (SEM) and MiSeq high-throughput sequencing technologies. The results indicated that when temperature increased from 15, 20, 25 to 30℃, the average TN removal rate of the system increased from 78.88% to 92.70%, the average removal rate of TP increased from 82.58% to 89.15%;microbial properties showed that the surface reaction after corncob was dominated by spherical and rod-shaped microorganisms; the proportion of cellulose-degrading bacteria was 11.01% higher at 30℃ than 20℃, and the proportion of denitrifying bacteria decreased by 21.26%. It can be seen that the cellulose -degrading bacteria were more sensitive to the temperature than the denitrification bacteria, and more obviously affected by the temperature. |
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