| 饥饿对硫自养反硝化反应器生物群落结构的影响 |
| 摘要点击 2269 全文点击 1190 投稿时间：2016-09-01 修订日期：2016-10-11 |
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| 中文关键词 硫自养反硝化 饥饿期 低温 MiSeq高通量测序 微生物多样性 群落结构 |
| 英文关键词 sulfur autotrophic denitrification starvation period low temperature MiSeq high throughput sequencing microbial diversity community structure |
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| 中文摘要 |
| 采用颗粒硫磺/白云石和黄铁矿/白云石自养反硝化反应器处理二沉尾水，探究饥饿忍耐对反应器去除效果、微生物群落变化的影响及其性能恢复时间.结果表明，在12~14℃的低温条件下，经过30 d的不进水饥饿忍耐后，颗粒硫磺/白云石和黄铁矿/白云石反应器出水NO3--N浓度分别从1.78 mg·L-1、11.32 mg·L-1增加到27.87 mg·L-1、26.56 mg·L-1.颗粒硫磺/白云石和黄铁矿/白云石自养反硝化反应器分别在重启后的第5 d和11 d使得NO3--N去除性能恢复至饥饿前的水平，并且在低温条件下保持了良好的脱氮效果.MiSeq高通量测序结果表明，2个反应器的细菌群落的丰度和多样性饥饿期均低于恢复期，且优势菌门均为Proteobacteria，主要的纲类为β-Proteobacteria.硫磺/白云石反应器中鉴别出Thiobacillus菌属为主要反硝化类群. |
| 英文摘要 |
| Sulfur/dolomite and pyrite/dolomite autotrophic denitrification reactors were applied to treat the secondary effluent of wastewater treatment plant to explore the removal effect, the changes of microbial community, and recovery time of reactors after starvation period. It was shown in the results that after 30 d non-water starvation endurance, the effluent concentrations of NO3--N in sulfur/dolomite and pyrite/dolomite reactors increased from 1.78 mg·L-1, 11.32 mg·L-1 to 27.87 mg·L-1, 26.56 mg·L-1 respectively at the low temperature of 12-14℃. In addition, sulfur/dolomite and pyrite/dolomite reactors recovered within 5 d and 11 d since restarted and could maintain a good effect of nitrogen removal at low temperature. MiSeq high throughput sequencing results showed that the abundance and diversity of the bacterial communities in starvation period in both reactors were lower than those in recovery period. The dominating phylum was Proteobacteria in both reactors while the dominating class was β-Proteobacteria. Thiobacillus was identified as the main genus for denitrification in sulfur/dolomite reactor. |
