| 微生物修复油污土壤过程中氮素的变化及菌群生态效应 |
| 摘要点击 4297 全文点击 1613 投稿时间：2016-08-01 修订日期：2016-09-21 |
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| 中文关键词 石油污染土壤 微生物修复 GC-MS MiSeq高通量测序 不同形态氮 |
| 英文关键词 petroleum contaminated soil bioremediation GC-MS Illumia MiSeq different forms of nitrogen |
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| 中文摘要 |
| 对陕北地区石油污染土壤进行了微生物修复研究，利用气相色谱-质谱联用技术（gas chromatography-mass spectrometry，GC-MS）测定土壤中烷烃和多环芳烃的含量变化，采用高通量测序技术研究微生物修复对油污土壤微生物群落多样性的影响，对修复过程中土壤不同形态氮含量变化进行了分析测定.结果表明，经过18周的修复，土壤中烷烃含量由初始时的25987.8 mg·kg-1降低为12788.6 mg·kg-1，多环芳烃含量由初始时的5322.9 mg·kg-1降低为2917.2mg·kg-1.高通量测序结果表明，经过微生物修复处理的土壤微生物群落结构发生了较大变化，一些可降解烃类的菌群如厚壁菌门（Firmicutes）、拟杆菌门（Bacterodetes）、迪茨氏菌属（Dietzia）、不动杆菌属（Acinetobacter）所占丰度增加.经过修复处理的土壤中总氮、氨氮含量呈降低趋势，硝态氮含量在修复前期呈降低趋势，修复后期基本保持不变.研究结果表明，微生物修复处理可去除土壤中的大部分烷烃和多环芳烃，烷烃和多环芳烃的降解效果与土壤中硝氮含量和烃降解菌所占丰度相关. |
| 英文摘要 |
| A laboratory study was conducted to investigate the impacts of bioremediation on microbial communities and various nitrogen shifts in petroleum contaminated soil by using GC-MS and Illumia MiSeq technique. Results showed the concentrations of alkane reduced from 25987.8 mg·kg-1 to 12788.6 mg·kg-1, and the concentrations of polycyclic aromatic hydrocarbons (PAHs) decreased from 5322.9 mg·kg-1 to 2917.2 mg·kg-1. Illumia MiSeq results showed that soil microbial communities shifted significantly after remediation, and the relative abundance of some phylum of hydrocarbon degraders (Firmicutes, Bacterodetes), and some genus of degraders (Dietzia, Acinetobacter) increased. Besides, the contents of total nitrogen and ammonia nitrogen increased firstly and then decreased during remediation. However, the contents of nitrate nitrogen decreased at the early stage, and then kept stable in the later stage of remediation. It can be concluded that bioremediation effectively promoted petroleum hydrocarbon degradation, and the different fractional hydrocarbon degradation was related to the relative abundance of hydrocarbon degraders and available nitrogen contents. |
