| 应用于矿山修复的高效菌株鉴定与溶岩机制:基于增强回归树分析 |
| 摘要点击 3254 全文点击 1130 投稿时间：2016-07-12 修订日期：2016-08-17 |
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| 中文关键词 硅酸盐岩 生物风化 假单胞菌 岩石矿区 生态修复 |
| 英文关键词 silicate rock biological weathering Pseudomonas sp. rock mining area ecological restoration |
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| 中文摘要 |
| 岩石矿区废弃地水土流失问题极度严峻，微生物对修复该问题造成的退化生境具有重要意义，为探究微生物对岩石矿区生境修复的有效性与作用机制，采用两次定向筛选，获得1株高效产铁载体及吲哚乙酸（IAA）的硅酸盐矿物分解细菌，利用16S rRNA基因序列分析鉴定该细菌为Pseudomonas protegens.通过该细菌对硅酸盐岩的岩石分解实验，测定培养液pH值，K、Al、Si这3种元素的释放量，检测岩石溶蚀的粒径变化，并分析培养液中不同有机酸，氨基酸及多糖的浓度变化，研究Pseudomonas protegens NLX-4对硅酸盐的溶蚀效果，同时引入增强回归树（boosted regression tree analysis，BRT）分析，探究其促溶机制.分析表明，该细菌通过代谢积累一定浓度的酒石酸（>777 mg·L-1）以及多糖（>8.21g·L-1），对硅酸盐岩产生释钾、释铝、脱硅的作用，从而有效促进岩石的分解.菌株Pseudomonas protegens NLX-4可作为修复废弃矿地被破坏生境的良好菌种资源. |
| 英文摘要 |
| The soil and water loss problem in rock mining areas is an extremely serious problem,and microbes play significant roles in ecological restoration of those areas.In this study,directive screening was used to explore the efficiency of microbe-mediated habitat restoration and the underlying mechanisms.A bacterial strain NLX-4,which was then identified as Pseudomonas protegens according to its 16S rRNA gene sequence,was screened out as an efficient silicate dissolution bacterium with the ability to secrete siderophore and indole-3-acetic acid (IAA).Moreover,pH value,element (K,Al,Si) release,organic acid content,amino acid concentration,polysaccharide content,and rock particle diameter variation in culture medium were analyzed to explore the ability of P.protegens NLX-4 to promote dolomite dissolution under controlled experimental conditions.These results showed that P.protegens NLX-4 could play a positive role in dolomite dissolution by producing tartaric acid (>777 mg·L-1) and polysaccharides (>8.21g·L-1).Therefore,P.protegens NLX-4 is an efficient microbial resource that can be used in rehabilitation of abandoned mines and has great application potential. |
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