| 活性污泥厌氧Fe(Ⅲ)还原氨氧化现象初探 |
| 摘要点击 3822 全文点击 1511 投稿时间：2016-03-19 修订日期：2016-04-01 |
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| 中文关键词 活性污泥 厌氧环境 Fe(Ⅲ)还原氨氧化 变性梯度凝胶电泳(DGGE) 微生物群落 |
| 英文关键词 activated sludge anaerobic environment ferric reduction and ammonia oxidation denaturing gradient gel electrophoresis (DGGE) microbial community |
| 作者 | 单位 | E-mail | | 李祥 | 苏州科技大学环境科学与工程学院, 苏州 215009
苏州科技大学环境生物技术研究所, 苏州 215009 | lixiang@mail.usts.edu.cn | | 林兴 | 苏州科技大学环境科学与工程学院, 苏州 215009
苏州科技大学环境生物技术研究所, 苏州 215009 | | | 杨朋兵 | 苏州科技大学环境科学与工程学院, 苏州 215009
苏州科技大学环境生物技术研究所, 苏州 215009 | | | 黄勇 | 苏州科技大学环境科学与工程学院, 苏州 215009
苏州科技大学环境生物技术研究所, 苏州 215009 | | | 刘恒蔚 | 苏州科技大学环境科学与工程学院, 苏州 215009
苏州科技大学环境生物技术研究所, 苏州 215009 | |
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| 中文摘要 |
| 采用常规化学分析和微生物群落变性梯度凝胶电泳（DGGE）监测技术，探究了厌氧条件下活性污泥中Fe（Ⅲ）还原氨氧化（Feammox）反应的存在及微生物群落动态响应. 结果表明，当反应器运行至第24 d时NH4+发生转化，同时检测到NO3-和Fe（Ⅱ）的生成，表明活性污泥中存在着Fe（Ⅲ）还原NH4+氧化反应，产物主要为NO3-和Fe（Ⅱ），并伴随少量N2生成. 经过84 d培养，氨氮最大转化量达29.85 mg·L-1，转化率为59.7%，出水NO3-最高值达24.56 mg·L-1. 活性污泥中Feammox为产酸过程，体系中pH值下降. 整个培养过程中微生物群落条带分布发生变化，参与活性污泥中Feammox反应的部分群落在培养过程获得保留，部分优势菌群获得富集. |
| 英文摘要 |
| In recent years, a few phenomena of ferric reduction and ammonia oxidation (Feammox) have been discovered in Paddy soil, lake sediments and wetland soil, but none was observed in activated sludge. Thus, the Feammox process and the dynamic response of corresponding microbial community in activated sludge were explored by conventional chemical analyses and microbial community denatured gradient gel electrophoresis (DGGE) technique. After 24 d of operation, ammonia transformation occurred. Nitrate and ferrous ion were detected in the reactor, indicating the existence of Feammox process in activated sludge with a reduction of nitrate and ferrous ion accompanied by a small amount of nitrogen gas. After 84 days of culture, the highest inversion of ammonia was 29.85 mg·L-1, the conversion rate of ammonia reached 59.7%, and the highest nitrate concentration of the reactor effluent was 24.56 mg·L-1. Feammox in activated sludge was a process that resulted in acid leading, which decreased the pH value. The structure of community bands changed during the whole incubation, some communities were retained and part of the dominant bacteria were enriched in the reaction of activated sludge. |
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