| 菹草乙酸乙酯组分抑藻活性物质的分离纯化和鉴定 |
| 摘要点击 3121 全文点击 1766 投稿时间：2015-04-09 修订日期：2015-05-15 |
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| 中文关键词 赤潮微藻 分离 鉴定 抑藻活性物质 菹草 |
| 英文关键词 red tide microalgae isolation identification antialgal activity substances Potamogeton crispus |
| DOI 10.13227/j.hjkx.20151040 |
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| 中文摘要 |
| 前期研究表明,菹草乙酸乙酯组分能显著抑制赤潮微藻米氏凯伦藻的生长. 在此基础上,采用硅胶柱层析和硅胶GF254薄层层析等方法,分离纯化菹草乙酸乙酯组分中的抑藻活性物质. 进一步应用质谱、核磁共振碳谱和氢谱等技术,鉴定抑藻活性物质. 结果表明,从菹草乙酸乙酯组分中分离纯化到2种抑藻活性物质. 当浓度为16 μg·mL-1时,它们对米氏凯伦藻表现出一定的抑藻活性. 结构鉴定表明,此2种抑藻活性物质为Trichodermatides B和2-methylheptylisonicotinate. 此2种化合物为首次从菹草中分离得到,也是首次从沉水植物中分离得到. |
| 英文摘要 |
| Previous studies showed that ethyl acetate extracts from the submerged macrophytes Potamogeton crispus can significantly inhibit the growth of Karenia mikimitoi.Further, two antialgal activity compounds (1-2) were successfully isolated from this submerged macrophytes through a combination of silica gel column chromagraphy and repeated preparative thin-layer chromatography in this paper.These two antialgal activity compounds exhibited antialgal active against Karenia mikimitoi.Furthermore, their structure were identified on the basis of spectroscoic data: one flavonid named Trichodermatides B, and one alkaloid named 2-methylheptylisonicotinate.These two compounds were for the first time isolated from both Potamogeton crispus and submerged macrophytes. |
