| 水介质中C60纳米晶体颗粒与Cu2+对小鼠腹腔巨噬细胞的复合毒性研究 |
| 摘要点击 2806 全文点击 1161 投稿时间：2014-10-14 修订日期：2015-01-25 |
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| 中文关键词 C60 小鼠腹腔巨噬细胞 Cu2+ 吸附 细胞毒性 |
| 英文关键词 C60 mouse peritoneal microphage Cu2+ adsorption cytotoxicity |
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| 中文摘要 |
| 为考察C60纳米晶体颗粒和Cu2+复合体系的生物毒性,本文以小鼠腹腔巨噬细胞RAW264.7作为受试对象,研究了C60纳米晶体颗粒物(nC60)和Cu2+对RAW 264.7的复合毒性影响. 结果表明,当单独暴露nC60 24 h,nC60 浓度为6.6 mg ·L-1时,细胞活性降低了45%,nC60 浓度为9.9 mg ·L-1 时,细胞活性降低了70%,表现出剂量-毒性效应和时间-毒性效应. 水中出现Cu2+可以降低nC60的细胞毒性,当Cu2+浓度为2 mg ·L-1,nC60浓度为6.6 mg ·L-1时, 细胞的活性降低至25%,当Cu2+浓度为5 mg ·L-1,nC60 浓度为9.9 mg ·L-1时,细胞的活性下降至15%. 研究表明,nC60表面能够吸附Cu2+,并且吸附符合Langmuir吸附等温模型. nC60纳米晶体颗粒表面对Cu2+的吸附,可能是nC60对细胞毒性降低的重要原因. |
| 英文摘要 |
| This work evaluated the cytotoxicity effect of nC60 nano-crystal particle associated with Cu2+by using mouse peritoneal microphage RAW264.7 as the test cell line. The results showed that when exposed to nC60 of 6.6 mg ·L-1 and 9.9 mg ·L-1 for 24h, the cell viability decreased 45% and 70%, respectively, and had obvious time-response and dose-response. It was found that Cu2+ could reduce the toxicity of nC60 on RAW 264.7. The cell viability reduced to 25% when exposed to 6.6 mg ·L-1 nC60 with 2 mg ·L-1 Cu2+, and reduced to 15% when exposed to 9.9 mg ·L-1 nC60 with 5 mg ·L-1 Cu2+. The nC60 could adsorb Cu2+and the adsorption isotherm was fitted to the Langmuir adsorption isotherm. The adsorption of Cu2+ on the surface of nC60 may decrease the cytotoxicity nC60 on RAW 264.7. |
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