| 内源呼吸过程溶解性代谢产物的光谱特性分析 |
| 摘要点击 4152 全文点击 2093 投稿时间：2014-01-28 修订日期：2014-04-02 |
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| 中文关键词 内源呼吸 比呼吸速率 三维荧光光谱 紫外光谱 腐殖酸 |
| 英文关键词 endogenous respiration specific respiratory rate three-dimensional fluorescence spectroscopy ultraviolet spectroscopy humic acid |
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| 中文摘要 |
| 微生物内源呼吸期对污水处理过程具有重要意义. 为了更清晰地了解微生物内源呼吸过程，采用三维荧光光谱、紫外光谱结合呼吸图谱对微生物内源呼吸过程进行解析. 结果表明，三维荧光光谱和紫外光谱在内源呼吸各个阶段有明显的不同：1内源呼吸快速下降期（第1 d）：腐殖酸峰的激发波长（Ex）和发射波长（Em）分别出现5 nm、6 nm的蓝移，荧光指数f450/500和HIX（humification index）分别减小了9.3%、0.2%，UV253/203和UV254分别增加了37.5%、200%，预示着混合液中可生物降解有机物的存在；2内源呼吸慢速下降期（第2 d）：f450/500增加了0.5%，HIX减小0.2%，UV253/203减小了20%，UV254增加了16.7%，预示着细胞可能出现了水解或自溶；3内源呼吸稳定期（第3~5 d）：腐殖酸峰基本保持不变预示了微生物适应了饥饿环境. 本研究从代谢产物的角度解析了内源呼吸过程，为污水处理过程控制提供了新的思路. |
| 英文摘要 |
| Endogenous respiration phase plays an important role in the sewage treatment process. In order to clearly understand the endogenous respiration process of the activated sludge process, three-dimensional fluorescence spectroscopy, ultraviolet spectroscopy and respirogram were employed for the analysis of endogenous respiration process. Results showed that the three-dimensional fluorescence spectroscopy and UV spectroscopy could identify all stages significantly. The following conclusions could be drawn: 1Rapid decline phase of endogenous respiration:the excitation wavelength (Ex) and emission wavelength (Em) of humic peak showed blue shift of 5 nm and 6 nm, respectively, the fluorescence index f450/500 and HIX (humification index) were reduced by 9.3% and 0.2%, respectively, UV253/203 and UV254 increased by 37.5% and 200%, respectively. These results indicated the presence of bioavailable organics; 2Slow decline phase of endogenous respiration: f450/500 was increased by 0.5%, HIX was reduced by 0.2%, UV253/203 was reduced by 20%, UV254 was increased by 16.7%. These results indicated that hydrolysis or autolysis of cells might occur; 3Stable phase of endogenous respiration: humic acid peak remained unchanged, indicating the adaption of microorganisms to starving environment. The analysis of the endogenous respiration process from the perspective of metabolites provides a new way for control of microbial wastewater treatment process. |
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