| 黄河中下游典型抗性细菌及抗性基因污染分布 |
| 摘要点击 1196 全文点击 180 投稿时间：2022-11-29 修订日期：2023-04-17 |
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| 中文关键词 黄河流域 抗性基因（ARGs） 抗性细菌（ARB） 时空分布 荧光定量PCR（qPCR） |
| 英文关键词 Yellow River Basin antibiotic resistance genes（ARGs） antibiotic resistant bacteria（ARB） spatial and temporal distribution quantitative-polymerase chain reaction（qPCR） |
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| 中文摘要 |
| 以黄河中下游某城市段的黄河水体作为研究对象，对其中可培养总细菌、典型抗性细菌［阿莫西林（AMX）抗性细菌、磺胺甲唑（SMZ）抗性细菌］及其对应的典型抗性基因［β-内酰胺类抗性基因（blaCTX-M）、磺胺类抗性基因（sulI、sulⅡ）］、1种整合酶基因intⅠ1的季节及沿程分布进行调研分析.结果表明，该市黄河流域可培养总细菌、AMX抗性细菌和SMZ抗性细菌受温度与人类活动影响显著，其菌属组成与数量具有明显的时空分布特征，主要以芽孢杆菌属（Bacillus）和假单胞菌属（Pseudomonas）为主.抗性基因丰度随温度降低呈下降趋势，β-内酰胺类抗性基因在总基因中占比高于磺胺类基因，磺胺类基因中以sulI为优势基因.相关性分析表明，Ⅰ型整合子是加速抗性基因传播的重要因素.研究有助于了解该地黄河水体抗性污染现状，可为黄河中下游流域抗性基因风险评估提供理论支撑. |
| 英文摘要 |
| The Yellow River water of an urban area located in the middle and lower reaches of the Yellow River was taken as the research object， in which the seasonal and along-range distribution of total culturable bacteria， typical antibiotic resistant bacteria （amoxicillin resistant bacteria and sulfamethoxazole-resistant bacteria）， and their corresponding typical resistance genes ［β-lactam resistance gene （blaCTX-M） and sulfamamide resistance genes （sulI and sulⅡ）， as well as intⅠ1 were investigated. The results showed that the total culturable bacteria， β-lactam-resistant bacteria and sulfonamide-resistant bacteria in the Yellow River Basin were significantly affected by temperature and human activities. The composition and quantity of their genera had obvious spatiotemporal distribution characteristics， in which Bacillus and Pseudomonas were dominant in the composition and number of bacteria. The abundance of resistance genes decreased with the decrease in temperature. The proportion of β-lactam resistance genes in the total genes was higher than that of sulfanilamide genes， and sulI was the dominant gene in sulfanilamide genes. Correlation analysis showed that class Ⅰ integron played an important role in accelerating the spread of resistance genes. This study offers insight into the status quo of water resistance pollution in the Yellow River and provides theoretical support for the risk assessment of resistance genes in the middle and lower reaches of the Yellow River Basin. |
