| 叶面喷施硅肥对再生水灌溉水稻叶际细菌群落结构及功能基因的影响 |
| 摘要点击 653 全文点击 177 投稿时间：2023-02-24 修订日期：2023-03-24 |
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| 中文关键词 硅肥 再生水灌溉 叶际 细菌群落 功能基因 |
| 英文关键词 silicon fertilizer reclaimed water irrigation phyllosphere bacterial community functional genes |
| 作者 | 单位 | E-mail | | 梁胜贤 | 河北大学生命科学学院, 生命科学与绿色发展研究院, 保定 071000 | liangshengxiansr@163.com | | 刘春成 | 中国农业科学院农田灌溉研究所, 新乡 453002
中国农业科学院农业水资源高效安全利用重点开放实验室, 新乡 453002 | | | 胡超 | 中国农业科学院农田灌溉研究所, 新乡 453002
中国农业科学院农业水资源高效安全利用重点开放实验室, 新乡 453002 | | | 崔二苹 | 中国农业科学院农田灌溉研究所, 新乡 453002
中国农业科学院农业水资源高效安全利用重点开放实验室, 新乡 453002 | | | 李中阳 | 中国农业科学院农田灌溉研究所, 新乡 453002
中国农业科学院农业水资源高效安全利用重点开放实验室, 新乡 453002 | | | 樊向阳 | 中国农业科学院农田灌溉研究所, 新乡 453002
中国农业科学院农业水资源高效安全利用重点开放实验室, 新乡 453002 | | | 崔丙健 | 中国农业科学院农田灌溉研究所, 新乡 453002
中国农业科学院农业水资源高效安全利用重点开放实验室, 新乡 453002 | ayangcbj@126.com |
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| 中文摘要 |
| 再生水农业利用被认为是解决水资源短缺和减少水环境污染的有效途径.硅肥可提高作物产量及品质,并增强作物抗逆能力.叶面喷施硅肥对叶际微生物群落的影响仍缺少全面研究.通过盆栽试验,探讨了不同种类硅肥叶面喷施对再生水灌溉水稻叶际细菌群落结构组成及多样性和相关功能基因丰度的影响.结果表明,水稻叶际细菌优势菌门主要为厚壁菌门（Firmicutes）、变形菌门（Proteobacteria）、放线菌门（Actinobacteriota）、拟杆菌门（Bacteroidota）和疣微菌门（Verrucomicrobiota）.芽孢杆菌属（Bacillus）相对丰度较其他处理在再生水灌溉+叶面喷施那优硅（RIS3）处理中更高,再生水灌溉使潜在致病菌泛菌属（Pantoea）和肠杆菌属（Enterobacter）的相对丰度显著增加,其中未分类的菌属也是水稻叶际细菌群落重要的组成部分.硅肥处理显著富集了芽孢杆菌属（Bacillus）、微小杆菌属（Exiguobacterium）、Aeromonas和柠檬酸杆菌属（Citrobacter）.功能预测分析表明,指示物种主要参与代谢与降解功能,并且预测的功能类群主要来自化能异养、好氧化能异养、硝酸盐还原和发酵.定量PCR结果显示,各处理中氨氧化古菌（AOA）、氨氧化细菌（AOB）和固氮酶基因（nifH）均处于较低的丰度水平,亚硝酸还原酶基因（nirK）丰度在各处理间也无显著差异.上述研究结果有助于深层次了解叶面喷施硅肥影响水稻叶际细菌群落结构及多样性差异,为硅肥在再生水灌溉农业中的应用提供理论依据. |
| 英文摘要 |
| Agricultural utilization of reclaimed water is considered to be an effective way to solve water shortage and reduce water environmental pollution. Silicon fertilizer can improve crop yield and quality and enhance crop resistance. The effect of foliar spray with silicon fertilizer on phyllosphere microbial communities remains lacking. In this study, a pot experiment was conducted to explore the effects of different types of silicon fertilizer on the composition and diversity of a phyllosphere bacterial community and the abundances of related functional genes in rice irrigated with reclaimed water. The results showed that Firmicutes, Proteobacteria, Actinobacteriota, Bacteroidota, and Verrucomicrobiota dominated the phyllosphere bacteria of rice. The relative abundance of Bacillus was higher than that of other treatments in RIS3. Reclaimed water irrigation significantly increased the relative abundances of the potential pathogens Pantoea and Enterobacter. The unclassified bacteria were also an important part of the bacterial community in the rice phyllosphere. Bacillus, Exiguobacterium, Aeromonas, and Citrobacter were significantly enriched by silicon fertilizer treatments. Functional prediction analysis showed that indicator species were mainly involved in metabolism and degradation functions, and the predicted functional groups of phyllosphere bacteria were attributed to chemoheterotrophy, aerobic chemoheterotrophy, nitrate reduction, and fermentation. Quantitative PCR results showed that AOA, AOB, and nifH genes were at low abundance levels in all treatments, and nirK genes was not significantly different among treatments. These results contribute to the in-depth understanding of the effects of foliar spray silicon fertilizer on the bacterial community structure and diversity of rice phyllosphere and provide a theoretical basis for the application of silicon fertilizer in reclaimed water irrigation agriculture. |
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