| 海洋菌株y3的分离鉴定及其异养硝化-好氧反硝化特性 |
| 摘要点击 2760 全文点击 1151 投稿时间：2015-07-24 修订日期：2015-10-19 |
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| 中文关键词 海洋菌株 异养硝化 好氧反硝化 假单胞菌属 混合氮源 脱氮特性 |
| 英文关键词 marine bacterium heterotrophic nitrification aerobic denitrification Pseudomonas sp. mixed nitrogen sources nitrogen removal characteristics |
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| 中文摘要 |
| 从胶州湾海底沉积物中筛选出1株高效的海洋异养硝化-好氧反硝化细菌y3,经形态学观察、生理生化实验和16S rRNA基因序列分析,确定该菌株为假单胞菌属(Pseudomonas sp.). 对其在实际含氮海水中的脱氮实验结果表明,菌株y3的最佳碳源为柠檬酸三钠,最适pH为7.0,最适C/N为13; 菌株均能以NH4Cl、NaNO2和KNO3为唯一氮源进行反应,20 h后其去除率分别为98.69%、78.38%和72.95%,在硝化过程中几乎没有亚硝酸盐和硝酸盐的积累. 以不同比例混合两种氮源反应20 h,当NO3--N :NO2--N分别为2 :1、1 :1和1 :2时,脱氮率分别为99.56%、99.75%和99.41%; 当NH4+-N :NO3--N分别为2 :1、1 :1、1 :2时,脱氮率均为100%; 当NH4+-N :NO2--N分别为2 :1、1 :1、1 :2时,脱氮率分别为90.43%、92.79%和99.96%,多高于单一氮源的情况. 该菌株具有较好的高盐废水脱氮处理效能. |
| 英文摘要 |
| A heterotrophic nitrification - aerobic denitrification bacterium named y3 was isolated from the sludge of Jiaozhou Bay using the enrichment medium with seawater as the matrix. It was identified as Pseudomonas sp. based on the morphological observation, physiological experiments and sequence analysis of 16S rRNA. The experiment results showed that the optimal carbon resource was sodium citrate, the optimal pH was 7.0, and the optimal C/N was 13. The strain could use NH4Cl, NaNO2 and KNO3 as sole nitrogen source, and the removal efficiencies were 98.69%, 78.38% and 72.95% within 20 hours, respectively. There was no nitrate and nitrite accumulation during the heterotrophic nitrification process. Within 20 hours, the nitrogen removal efficiencies were 99.56%, 99.75% and 99.41%, respectively, in the mixed system with NO3--N :NO2--N of 2 :1, 1 :1 and 1 :2. When the NH4+-N :NO3--N ratios were 2 :1, 1 :1, 1 :2, the nitrogen removal efficiencies were all 100%. When the NH4+-N :NO2--N ratios were 2 :1,1 :1,1 :2, the nitrogen removal efficiencies were 90.43%, 92.79% and 99.96%, respectively. They were higher than those with single nitrogen source. As a result, strain y3 had good nitrogen removal performance in high saline wastewater treatment. |
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