| 缙云山马尾松林和柑橘林土壤微生物PLFA沿海拔梯度的变化 |
| 摘要点击 2874 全文点击 1323 投稿时间：2015-03-21 修订日期：2015-07-13 |
| 查看HTML全文
查看全文 查看/发表评论 下载PDF阅读器 |
| 中文关键词 马尾松林 柑橘林 PLFA 土壤酶 海拔 |
| 英文关键词 masson pine citrus forest PLFA soil enzyme activity altitude |
| 作者 | 单位 | E-mail | | 曾清苹 | 西南大学资源环境学院, 三峡库区生态环境教育部重点实验室, 重庆 400715 | 709904956@qq.com | | 何丙辉 | 西南大学资源环境学院, 三峡库区生态环境教育部重点实验室, 重庆 400715 | hebinghui@swu.edu.cn | | 毛巧芝 | 西南大学资源环境学院, 三峡库区生态环境教育部重点实验室, 重庆 400715 | | | 吴耀鹏 | 西南大学资源环境学院, 三峡库区生态环境教育部重点实验室, 重庆 400715 | | | 黄祺 | 西南大学资源环境学院, 三峡库区生态环境教育部重点实验室, 重庆 400715 | | | 李源 | 西南大学资源环境学院, 三峡库区生态环境教育部重点实验室, 重庆 400715 | |
|
| 中文摘要 |
| 土壤微生物群落是土壤生态系统的重要组成部分,对环境变化敏感. 本文运用磷脂脂肪酸法(PLFA)研究缙云山马尾松林和柑橘林土壤微生物群落结构沿海拔梯度的变化特征. 结果表明,从6个海拔土壤中共检测到48种PLFA,其中i16:0、10Me17:0、10Me18:0 TBSA在6个海拔中含量均最高,且柑橘林土壤PLFA种类和含量明显高于马尾松林. 随着海拔升高马尾松林土壤微生物种类和含量逐渐增加,柑橘林则逐渐降低,各海拔间细菌、真菌、放线菌、革兰氏阴性菌(G-)及革兰氏阳性菌(G+)含量差异显著. 土壤微生物群落多样性研究结果表明,马尾松林低海拔丰富度指数(R),多样性指数(H')、均匀度指数(J)均显著高于高海拔,而柑橘林R在低海拔最高,H'、J则在高海拔最高. 不同海拔土壤细菌、放线菌、G-及G+与土壤酶和环境因子之间存在相关性,细菌、放线菌、G-及G+与脲酶(Ure)、转化酶(Ive)、过氧化氢酶(CAT)、林分类型均呈极显著正相关,真菌与Ure、Ive、CAT呈显著正相关; 而细菌、真菌、放线菌、G-及G+与海拔呈极显著或显著负相关. Ure、Ive、CAT、林分及海拔是影响土壤微生物PLFA变化的重要因子. |
| 英文摘要 |
| The soil microbial community is an important part in soil ecosystem, and it is sensitive to the ecological environment. Phospholipid-derived fatty acids(PLFA) analysis was used to examine variations in soil microbial community diversity and its influencing factors. The results showed that: there existed 48 PLFAs that were significant in the soil samples from six altitudes. The PLFAs of six altitudes with the highest contents were i16:0, 10Me17:0, 10Me18:0 TBSA. The citrus forest exhibited richer soil PLFAs distribution both in type and amount than those in masson pine. The microbial activity and functional diversity of masson pine were increased with increasing altitudes, and citrus forest gradually decreased, the PLFA content of different microbial groups in each altitude were significantly different. The richness index, Shannon-Wiener index and Pielou evenness index of masson pine in low elevation were holistically higher than those in high elevation. However, the highest richness index of citrus forest was in low altitude, the highest Shannon-Wiener index and Pielou evenness index were in high altitude. The PLFAs content of different microbial groups were closely correlated to the soil enzyme activities and environmental factors. The PLFAs of bacteria, actinomycetes, G-(Gram-positive), G+(Gram-negative) were positively correlated with Ure(urease), Ive(invertase), CAT(catalase activity) and forest type, the PLFAs of fungi was significantly correlated with Ure, Ive, CAT, the PLFAs of bacteria, fungi, actinomycetes, G-, G+ were significantly negatively or less correlated with elevation. Ure, Ive, CAT, forest type and elevation are the pivotal factors controlling the soil microbial biomass and activities. |
|
|
|
