| 3种PAEs对蚯蚓的毒性作用和组织酶活性影响的研究 |
| 摘要点击 2765 全文点击 1105 投稿时间：2013-03-13 修订日期：2013-07-04 |
| 查看HTML全文
查看全文 查看/发表评论 下载PDF阅读器 |
| 中文关键词 邻苯二甲酸酯类 赤子爱胜蚓 污染胁迫 急性毒性 抗氧化酶 |
| 英文关键词 phthalate acid esters Eisenia foetida pollution threat acute toxicity antioxidant enzymes |
|
| 中文摘要 |
| 以赤子爱胜蚓（Eisenia foetida）为受试生物，通过滤纸接触法和自然土壤法研究邻苯二甲酸二甲酯（Dimethyl Phthalate，DMP）、邻苯二甲酸二乙酯（Diethyl Phthalate，DEP）和邻苯二甲酸二正丁酯（Di-n-butyl Phthalate，DBP）的急性毒性效应. 结果表明，DEP和DMP对赤子爱胜蚓毒性显著，滤纸接触法染毒48 h时，DMP和DEP的半数致死剂量（LD50）分别为129.603 μg·cm-2和145.336 μg·cm-2. 自然土壤法染毒14 d时，DMP和DEP的LC50分别为1560.120 mg·kg-1和1516.186 mg·kg-1. DBP 的LD50尚无确切数据. 自然土壤法研究DMP、DEP与DBP对蚯蚓组织超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和乙酰胆碱酯酶（AChE）活性的影响. 结果表明，SOD、CAT和AChE活性对DMP、DEP和DBP的毒性响应各不相同，其中，DMP、DEP和DBP的浓度对SOD、CAT和AChE活性呈显著性影响. SOD活性被DMP和DEP诱导增加. CAT的活性在DEP和DBP低浓度下诱导高浓度作用下抑制，而AChE活性在DEP和DBP低浓度下抑制高浓度下诱导. 处理时间对SOD、AChE活性无显著性影响. |
| 英文摘要 |
| The acute toxic effects of DMP, DEP and DBP to earthworms (Eisenia foetida) were examined in this study. The two pollutants are tested by filter paper contact method and natural soil method, results indicated that they were both poisonous to earthworms. Earthworms were exposed to DMP and DEP for 48 h by filter paper contact method, LC50(DMP) is 129.603 μg·cm-2and LC50(DEP) is 145.336 μg·cm-2. Earthworms were exposed to DMP and DEP for 14 d by natural soil method, LC50(DMP) is 1560.120 mg·kg-1 and LC50(DEP) is 1516.186 mg·kg-1. While non-calculatable for the LC50 of DBP due to its low water solubility, penetrating into the skin and cells of earthworm is not easy. The natural soil test method was used to study the effects of DMP,DEP and DBP on enzyme activities of earthworms, activities of superoxide dismutase (SOD), catalase (CAT) and acetylcholinesterase (AChE) were measured. SOD activity was induced by DMP and DEP, CAT is the most sensitive and the activity was induced by DEP and DBP at the low concentration and inhibited at the high concentration. AChE activity was inhibited by DEP and DBP at the low concentration and induced at the high concentration, finally exhibited a tendency to recover to the control level at 28 d. |
