| 限NH4+和限NO2-厌氧氨氧化系统的运行特性及微生物种群特征 |
| 摘要点击 1510 全文点击 798 投稿时间：2020-05-14 修订日期：2020-06-08 |
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| 中文关键词 厌氧氨氧化(ANAMMOX) 空间异质性 颗粒污泥 絮体 主流 |
| 英文关键词 ANAMMOX spatial heterogeneity granular sludge flocs mainstream |
| 作者 | 单位 | E-mail | | 宋家俊 | 苏州科技大学环境科学与工程学院, 苏州 215009 | 875078465@qq.com | | 朱明奕 | 苏州科技大学环境科学与工程学院, 苏州 215009 | | | 郭凯成 | 苏州科技大学环境科学与工程学院, 苏州 215009 | | | 汪倩 | 苏州科技大学环境科学与工程学院, 苏州 215009 | | | 刘文如 | 苏州科技大学环境科学与工程学院, 苏州 215009
城市生活污水资源化利用技术国家地方联合工程实验室, 苏州 215009
江苏省水处理技术与材料协同创新中心, 苏州 215009 | liuwenru1987@126.com | | 沈耀良 | 苏州科技大学环境科学与工程学院, 苏州 215009
城市生活污水资源化利用技术国家地方联合工程实验室, 苏州 215009
江苏省水处理技术与材料协同创新中心, 苏州 215009 | |
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| 中文摘要 |
| 在控制进水TN浓度<50 mg·L-1、水力停留时间为2.0 h和水温为20℃条件下，采用连续流完全混合式反应器对比研究了限NH4+和限NO2-厌氧氨氧化系统的脱氮效能及微生物种群特征.结果表明，尽管两厌氧氨氧化反应器维持了类似的TN去除负荷［0.45~0.5 kg·（m3·d）-1］和TN去除率（70%左右），但限NH4+厌氧氨氧化反应器中ΔNO3-/ΔNH4+呈现更快的上升趋势.批式试验及高通量测序结果表明，限NH4+厌氧氨氧化反应器比限NO2-反应器具有更为显著的功能及微生物种群空间异质性.Candidatus_Brocadia是两反应器中的优势厌氧氨氧化菌属，限NH4+条件下Candidatus_Brocadia在大粒径颗粒污泥中的富集水平（53.9%）显著高于絮体污泥（19.1%）；而在限NO2-条件下颗粒污泥与絮体污泥中Candidatus_Brocadia的相对丰度差别不大，分别为28.1%和21.3%.两反应器中均有Nitrospira存在且主要生存于絮体污泥中，对O2的需求应是驱动Nitrospira于絮体污泥中生长的关键因素；此外，限NH4+（即NO2-富余）环境可以促进Nitrospira的生长繁殖.综上，提出了基于选择性排泥的限NH4+厌氧氨氧化系统优化运行策略. |
| 英文摘要 |
| The performance and microbial characteristics of ammonium-limited and nitrite-limited ANAMMOX reactors were studied in two continuously stirred tank reactors. The influent TN concentrations were controlled below 50 mg·L-1. The hydraulic retention time and water temperature were maintained at 2.0 h and 20℃, respectively. Results showed that though both ANAMMOX reactors demonstrated similar TN removal loading rates[0.45-0.5 kg·(m3·d)-1] and TN removal efficiencies (around 70%), the ΔNO3-/ΔNH4+ ratio of the ammonium-limited ANAMMOX reactor showed a faster upward trend. Batch tests and high-throughput sequencing results indicated that the ammonium-limited ANAMMOX reactor had more significant functional and population heterogeneity than the nitrite-limited ANAMMOX reactor. Candidatus_Brocadia was the predominant ANAMMOX bacteria in both reactors. The relative abundance of Candidatus_Brocadia in large granules (53.9%) was significantly higher than that in flocs (19.1%) under the ammonium-limited conditions, whereas only a small difference in relative abundance of Candidatus_Brocadia was observed between the granules (28.1%) and flocs (21.3%) in the nitrite-limited ANAMMOX reactor. Nitrospira-like NOB were detected in both ANAMMOX reactors, which primarily inhabited flocs, seemingly driven by the availability of oxygen. Moreover, the ammonium-limited (i.e., excess nitrite) conditions seemingly favored the growth of Nitrospira. Building upon these results, a control strategy for optimal operation of the ammonium-limited ANAMMOX reactor was proposed based on selective floc discharge. |
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