| 分层型水库藻类垂向演替的水质与细菌种群调控 |
| 摘要点击 1462 全文点击 556 投稿时间：2020-01-14 修订日期：2020-01-20 |
| 查看HTML全文
查看全文 查看/发表评论 下载PDF阅读器 |
| 中文关键词 分层型水库 藻类 细菌种群代谢 高通量DNA测序 种群垂向演替 |
| 英文关键词 stratified reservoir algae bacterial community metabolism high-throughput DNA sequencing community vertical succession |
| 作者 | 单位 | E-mail | | 闫苗苗 | 西安建筑科技大学环境与市政工程学院, 陕西省环境工程重点实验室, 西北水资源与环境生态教育部重点实验室, 西安 710055 | Ymmzsj@163.com | | 陈胜男 | 西安建筑科技大学环境与市政工程学院, 陕西省环境工程重点实验室, 西北水资源与环境生态教育部重点实验室, 西安 710055 | chenshengnan@xauat.edu.cn | | 黄廷林 | 西安建筑科技大学环境与市政工程学院, 陕西省环境工程重点实验室, 西北水资源与环境生态教育部重点实验室, 西安 710055 | huangtinglin@xauat.edu.cn | | 贾竞宇 | 西安建筑科技大学环境与市政工程学院, 陕西省环境工程重点实验室, 西北水资源与环境生态教育部重点实验室, 西安 710055 | | | 刘凯文 | 西安建筑科技大学环境与市政工程学院, 陕西省环境工程重点实验室, 西北水资源与环境生态教育部重点实验室, 西安 710055 | | | 苗雨甜 | 西安建筑科技大学环境与市政工程学院, 陕西省环境工程重点实验室, 西北水资源与环境生态教育部重点实验室, 西安 710055 | | | 宗容容 | 西安建筑科技大学环境与市政工程学院, 陕西省环境工程重点实验室, 西北水资源与环境生态教育部重点实验室, 西安 710055 | |
|
| 中文摘要 |
| 浮游藻类和细菌是水生食物网的重要组成部分，在水源水库生态系统物质循环和能量流动方面发挥关键作用，藻类的空间演替与水体细菌种群代谢和结构演变关系密切.因此，本文以李家河水库为研究对象，在分析水库水质指标基础上，采用高通量DNA测序技术和Biolog技术，研究水库藻类暴发期，浮游藻类和细菌群落结构垂向演替特征及其与水质的偶联关系.结果表明，李家河水库在8月处于热分层期，水体pH、DO和NH4+-N随深度变化均逐渐降低（P<0.001）.藻密度和Chla呈现同步变化趋势（P<0.001），表层最大分别为3364.33×104 cells·L-1和7.03 μg·L-1.藻类群落结构在水深0 m和3 m处以微囊藻为主，而在水深6 m处，小环藻取代微囊藻成为最优藻属，相对丰度达57.28%.Biolog结果表明，微囊藻的暴发对细菌代谢活性及其相对丰度产生较大影响，但细菌种群代谢活性多样性变化不显著.高通量测序共发现1420个OTUs，隶属于10个细菌门类，其中放线菌门（Actinobacteria）和变形菌门（Proteobacteria）在不同水层均为最优门类，其相对丰度之和达50%以上；绿菌门（Chlorobi）和浮霉菌门（Planctomycetes）的相对丰度随着水深增加发生显著变化，均在6 m深度达到最大值，分别为10.29%和6.78%，且与水层藻密度呈显著负相关（P<0.05）；厚壁菌门（Firmicutes）和芽单胞菌门（Gemmatimonadetes）与藻密度呈显著正相关（P<0.05）.热图（Heat map）指纹图谱表明，李家河水库的细菌群落结构垂向分布差异显著，且随着水深的增加，细菌群落分布更均匀并趋于多样性.冗余分析（RDA）表明，细菌和藻类的群落结构的垂向分布受不同水质指标调控且差异显著.对水源水库藻类暴发期藻类与细菌群落的偶联机制进行探究，为水源藻华消涨的分子微生态驱动机制研究提供科学依据. |
| 英文摘要 |
| Phytoplankton and bacteria are important components of the aquatic food web, and play a critical role in substance circulation and energy exchange in freshwater ecosystems. The succession of algae is closely related to the metabolism and structural succession of bacterial populations in the water column. Thus, in this study, the vertical succession characteristics of phytoplankton and bacteria community structure and their coupling with water quality were investigated during an algal bloom in the Lijiahe Reservoir using high-throughput DNA sequencing and Biolog technologies. The results showed that the Lijiahe Reservoir was in the thermal stratification stage in August, and the pH, dissolved oxygen, and NH4+-N of the water column gradually decreased with depth (P<0.001). Algal cell concentration and chlorophyll a exhibited a simultaneous trend (P<0.001), and the maximum values in the surface layer were 3363.33×104 cells·L-1 and 7.03 μg·L-1, respectively. The algal community structure was dominated by Microcystis at water depths of 0 m and 3 m, and at 6 m water depth, Cyclotella replaced Microcystis as the most dominant algae, with a relative abundance of 57.28%. Biolog analysis indicated that the outbreak of Microcystis had a significant impact on bacterial metabolic activity and its relative abundance, but the diversity of bacterial population metabolic activity varied less. A total of 1420 operational taxonomic units were found by high-throughput sequencing, belonging to 10 bacterial phyla. Of these, Actinobacteria and Proteobacteria dominated in all water layers, and their relative abundances were more than 50%. The relative abundance of Chlorobi and Planctomycetes varied significantly with water depth, reaching their maxima at a depth of 6 m with values of 10.29% and 6.78%, respectively, which were both negatively correlated with algal density (P<0.05). Firmicutes and Gemmatimonadetes were positively correlated with algal density (P<0.05). A heat map fingerprint showed that the vertical distribution of the bacterial community structure of the Lijiahe Reservoir varied significantly, and with the increase in water depth, the bacterial community was more uniformly distributed and tended to diversify. Redundancy analysis (RDA) showed that the vertical distribution of the bacterial and algal community structure was regulated by different water qualities, and the difference was significant. This study investigated the coupling mechanism of algal and bacterial communities during the algal bloom in the Lijiahe Reservoir, and the results provided a scientific basis for the investigation of the molecular microecological driving mechanism of water-source algal blooms. |
|
|
|
