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林可霉素菌渣堆肥微生物群落多样性分析
摘要点击 87  全文点击 35  投稿时间:2017-11-22  修订日期:2018-04-02
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中文关键词  堆肥  林可霉素菌渣  污泥  降解  高通量测序
英文关键词  composting  lincomycin mycelia dreg  sludge  degradation  high throughput sequencing
作者单位E-mail
任省涛 郑州大学化工与能源学院, 郑州 450001 273149687@qq.com 
郭夏丽 郑州大学化工与能源学院, 郑州 450001  
芦阿虔 郑州大学化工与能源学院, 郑州 450001  
张倩倩 郑州大学化工与能源学院, 郑州 450001  
郭笑盈 郑州大学化工与能源学院, 郑州 450001  
王岩 郑州大学化工与能源学院, 郑州 450001 wangyan371@zzu.edu.cn 
王连忠 河南省新乡华星制药厂, 新乡 453731  
张宝宝 河南省新乡华星制药厂, 新乡 453731  
中文摘要
      本试验以林可霉素菌渣-猪粪为原料、污泥-猪粪堆肥作对照,研究了林可霉素菌渣堆肥过程中残留林可霉素的降解情况,并基于Illumina MiSeq高通量测序分析了林可霉素菌渣堆肥过程中微生物菌群的变化.结果表明:通过堆肥处理可以大幅度降解林可霉素菌渣中残留的林可霉素,经过33 d的堆肥处理后,林可霉素的残留量从最初的1800 mg·kg-1降到483 mg·kg-1,降解率高达73%.同时高通量测序结果表明,由于高含量的林可霉素残留,在堆肥初期和高温期林可霉素菌渣堆肥中细菌群落的分布丰度和多样性指数均低于污泥-猪粪堆肥,但真菌群落丰度和多样性均高于污泥-猪粪堆肥.林可霉素菌渣堆肥中细菌主要以PaucisalibacillusCerasibacillusBacillusVirgibacillusUreibacillusPaenibacillusSinibacillus属为主,而污泥-猪粪堆肥中主要以TrueperaActinomaduraPseudosphingobacteriumPseudomonasLuteimonasUreibacillus属为主,两者堆肥中微生物群落结构存在显著差异.随着堆肥进入腐熟期,林可霉素残留大幅度降解,抗生素对微生物的胁迫减小或解除,林可霉素菌渣-猪粪堆肥和污泥-猪粪堆肥相比,无论是细菌还是真菌,其微生物群落已逐渐趋同.表明堆肥处理可以大幅降解林可霉素残留,增加微生物多样性,有利于实现林可霉素菌渣无害化处理和资源化利用.
英文摘要
      Aerobic composting experiments were conducted using lincomycin mycelia wastes (dreg) and manure (T), using sewage sludge with manure as a control (CK). High performance liquid phase methods and high throughput sequencing were used to determine the concentration of lincomycin residue and to characterize the microbial community. The results showed that lincomycin was reduced significantly, with the concentration decreasing from 1800 mg·kg-1 to 483 mg·kg-1, accounting for 73% degradation. In addition, the bacterial community abundance and diversity indices were all lower than that of sludge-manure at the mesophilic and thermophilic phases, because of the high concentration of lincomycin residue in lincomycin mycelia dreg. By contrast, the fungal community abundance and diversity indices showed the reverse, due to the high content of organic matter and nitrogen in lincomycin mycelia dreg. Therefore, the microbial communities were greatly different between T and CK treatment with the domain genera of Paucisalibacillus, Cerasibacillus, Bacillus, Virgibacillus, Ureibacillus, Paenibacillus, and Sinibacillus in T compost and Truepera, Actinomadura, Pseudosphingobacterium, Pseudomonas, Luteimonas and Ureibacillus in CK compost. However, as the composting continued to a mature phase, most of the lincomycin was reduced, and the differences between the two microbial communities gradually decreased. This showed that composting could make lincomycin mycelia dreg harmless and could be used to turn it into a resource.

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