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基于MBR不同种泥短程硝化启动的微生物群落结构分析
摘要点击 124  全文点击 37  投稿时间:2018-02-05  修订日期:2018-03-30
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中文关键词  短程硝化  膜生物反应器  接种污泥  启动  微生物群落结构
英文关键词  shortcut nitrification  membrane bioreactor (MBR)  inoculated sludge  start-up  microbial community
作者单位E-mail
吴鹏 苏州科技大学环境科学与工程学院, 苏州 215009
城市生活污水资源化利用技术国家地方联合工程实验室, 苏州 215009
江苏省水处理技术与材料协同创新中心, 苏州 215009
江苏省环境科学与工程重点实验室, 苏州 215009 
wupengniu@126.com 
陈亚 苏州科技大学环境科学与工程学院, 苏州 215009  
张婷 苏州科技大学环境科学与工程学院, 苏州 215009  
沈耀良 苏州科技大学环境科学与工程学院, 苏州 215009
城市生活污水资源化利用技术国家地方联合工程实验室, 苏州 215009
江苏省水处理技术与材料协同创新中心, 苏州 215009
江苏省环境科学与工程重点实验室, 苏州 215009 
ylshenniu@126.com 
徐乐中 苏州科技大学环境科学与工程学院, 苏州 215009
城市生活污水资源化利用技术国家地方联合工程实验室, 苏州 215009
江苏省水处理技术与材料协同创新中心, 苏州 215009
江苏省环境科学与工程重点实验室, 苏州 215009 
 
中文摘要
      为明确膜生物反应器(MBR)接种不同污泥启动短程硝化前后微生物群落结构变化特征,采用MBR反应器分别接种硝化污泥(R1)、厌氧亚硝化污泥(R2)和1:1混合接种厌氧亚硝化污泥和反硝化污泥(R3),获取有利于实现快速短程硝化的污泥源.结果表明,结合间歇曝气和缩短水力停留时间(HRT),R1、R2与R3反应器分别耗时46 d、8 d和30 d成功启动短程硝化,R2反应器启动周期最短.稳定运行期内,R1、R2和R3反应器亚硝累积率平均为92%、93%和94%,R3反应器表现出更稳定的短程硝化性能.ACE、Chao、Shannon和Simpson指数结果表明,稳定运行后,R1和R2反应器微生物丰度和多样性水平均大幅低于接种污泥,R3反应器物种丰度略有减少而多样性水平变化不大.短程硝化成功启动后,3个反应器内的主要菌群为变形菌门(Proteobacteria)和拟杆菌门(Bacteroidetes),且主要脱氮功能菌变形菌门丰度相较于接种污泥均有提高.β-变形菌纲为3个反应器短程硝化系统的优势菌群,分别占比59.6%、63.6%和69.3%.R1、R2和R3反应器内的优势菌属均为亚硝化单胞菌属(Nitrosomonas),所占比例分别达12.8%、20.2%和19.7%.相比R1反应器,R2和R3反应器接种污泥内存在一定比例的亚硝化细菌,更有利于系统短程硝化的实现.
英文摘要
      In order to clarify the microbial community characteristics of the shortcut nitrification start-up with different inoculated sludges in the membrane bioreactor (MBR), the MBR was inoculated with nitrification sludge (R1), anaerobic nitrification sludge (R2) and 1:1 mixed inoculated anaerobic nitrification and denitrification sludge (R3). The results showed that the combination of intermittent aeration and shortened hydraulic retention time (HRT) successfully achieved the shortcut nitrification by R1, R2 and R3 reactors after 46 d, 8 d and 30 d respectively, with the R2 reactor exhibiting the shortest start-up period. During stable operations, the average nitrite accumulation rates of R1, R2 and R3 reactors were 92%, 93% and 94% respectively, and the R3 reactor showed a more stable shortcut nitrification. The results of analyses using ACE, Chao, Shannon, and Simpson diversity indices showed that the microbial abundance and diversity levels of R1 and R2 were significantly lower than that of the inoculant during the stable operation period, while the species abundance in the R3 reactor was slightly reduced and the diversity level was slightly changed. The main bacteria in the three reactors were Proteobacteria and Bacteroidetes after the successful start-up of shortcut nitrification, and the relative abundance of Proteobacteria was increased compared with the inoculated sludge. Proteobacteria were the main denitrifying bacteria, with β-Proteobacteria being the dominant bacteria of the shortcut nitrification system in the three reactors, accounting for 59.6%, 63.6% and 69.3% respectively. Through further analysis, the next dominant bacteria in R1, R2 and R3 were all Nitrosomonas, with 12.8%, 20.2% and 19.7% respectively. Compared with the R1 reactor, there was a certain proportion of shortcut nitrification bacteria in the sludge of the R2 and R3 reactors, which was more favorable to the operation of the shortcut nitrification system.

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