| 高含固污泥厌氧消化中蛋白质转化规律 |
| 摘要点击 3128 全文点击 1199 投稿时间：2017-09-27 修订日期：2017-12-14 |
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| 中文关键词 剩余污泥 高含固 厌氧消化 蛋白质 氨氮 |
| 英文关键词 waste activated sludge high solids anaerobic digestion protein ammonia nitrogen |
| 作者 | 单位 | E-mail | | 詹瑜 | 江南大学环境与土木工程学院, 无锡 214122
江苏省厌氧生物技术重点实验室, 无锡 214122 | zhanyu9210@163.com | | 施万胜 | 江南大学环境与土木工程学院, 无锡 214122
江苏省厌氧生物技术重点实验室, 无锡 214122 | | | 赵明星 | 江南大学环境与土木工程学院, 无锡 214122
江苏省厌氧生物技术重点实验室, 无锡 214122 | | | 许之扬 | 江南大学环境与土木工程学院, 无锡 214122
江苏省厌氧生物技术重点实验室, 无锡 214122 | | | 阮文权 | 江南大学环境与土木工程学院, 无锡 214122
江苏省厌氧生物技术重点实验室, 无锡 214122 | wqruanjn@gmail.com | | 宋联 | 无锡国联环保科技股份有限公司, 无锡 214131 | | | 朱葛 | 无锡国联环保科技股份有限公司, 无锡 214131 | |
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| 中文摘要 |
| 采用剩余污泥在含固率（total solid，TS）为12%条件下进行中温（37℃）厌氧消化，通过分析厌氧消化前后污泥蛋白质组分的变化情况，研究了高含固污泥厌氧消化中蛋白质的转化规律，探讨了高含固条件下污泥蛋白质转化效率较低的原因.结果表明，经过45 d的厌氧消化处理，污泥蛋白质的转化率为34.26%.污泥蛋白质转化效率较低的原因主要表现在：①高含固条件下污泥的传质较差；同时，污泥蛋白质经水解过程形成大量的氨氮，反应结束后污泥总氨氮（total ammonia nitrogen，TAN）质量浓度达到1201 mg·L-1，导致对厌氧消化过程，尤其对蛋白质的分解表现出一定的抑制作用；②三维荧光光谱（three-dimensional fluorescence spectroscopy，3D-EEM）分析表明，部分蛋白质向腐殖质类、富里酸类物质转化，从而更难分解；③通过二维电泳（two-dimensional electrophoresis，2-DE）-质谱（mass spectrometry，MS）分析发现，厌氧消化后污泥蛋白质的相对分子质量和等电点（isoelectric point，pI）降低；最终，污泥中残留的大部分蛋白质来源于微生物体内.由于微生物代谢能力随着厌氧消化过程的进行而减弱，难以继续利用这些蛋白质，或消化体系中不具备分解这些蛋白质的酶，从而限制了污泥中蛋白质的分解效率. |
| 英文摘要 |
| Dewatered waste sludge with a total solid (TS) concentration of 12% was used for mesophilic (37℃) anaerobic digestion (AD). The biotransformation mechanism of protein and the reason for the low conversion efficiency of protein under high solids AD was investigated by analyzing the variation of protein composition in the sludge before and after AD. The results showed that the conversion rate of protein in the sludge was 34.26% after 45 days of AD. The reason for the low efficiency of protein conversion was the poor mass transfer efficiency under the condition of high solids content and the large amount of ammonia nitrogen produced with the hydrolysis. After 45 days of AD, the total ammonia nitrogen (TAN) concentration reached 1201 mg·L-1, which resulted in the inhibition of the AD process, especially the decomposition of protein. Some of the protein converted to humic acid-like and fulvic acid-like substances, which are more difficult to decompose based on the three-dimensional fluorescence spectroscopy (3D-EEM) analysis. Two-dimensional electrophoresis (2-DE)- mass spectrometry (MS) was adopted for identifying the composition of protein in sludge before and after AD. It showed that the relative molecular weight and the isoelectric point (pI) of the protein in the sludge decreased after AD and most of the proteins left in the digested sludge came from the micro-organisms. These proteins cannot be further decomposed by the microbes because of the decreased microbial metabolic capacity at the end of the AD process or lack of specific enzymes for the hydrolysis of these proteins. This ultimately resulted in the low decomposition efficiency of the total protein in the sludge. |
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