| 1株Arthrobacter arilaitensis菌的耐冷异养硝化和好氧反硝化作用 |
| 摘要点击 3552 全文点击 1398 投稿时间：2015-10-15 修订日期：2015-11-05 |
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| 中文关键词 异养硝化作用 阿氏节杆菌 好氧反硝化 氮转化 耐冷 |
| 英文关键词 heterotrophic nitrification Arthrobacter arilaitensis aerobic denitrification nitrogen conversion hypothermia |
| 作者 | 单位 | E-mail | | 何腾霞 | 西南大学资源环境学院, 土壤多尺度界面过程与调控重庆市重点实验室, 重庆 400716 | hetengxia5153@163.com | | 倪九派 | 西南大学资源环境学院, 土壤多尺度界面过程与调控重庆市重点实验室, 重庆 400716 | | | 李振轮 | 西南大学资源环境学院, 土壤多尺度界面过程与调控重庆市重点实验室, 重庆 400716 | lizhlun4740@sina.com | | 孙权 | 贵州省德江县中等职业学校, 德江 565200 | | | 冶青 | 西南大学资源环境学院, 土壤多尺度界面过程与调控重庆市重点实验室, 重庆 400716 | | | 徐义 | 西南大学资源环境学院, 土壤多尺度界面过程与调控重庆市重点实验室, 重庆 400716 | |
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| 中文摘要 |
| 分别采用高浓度的铵态氮、硝态氮、亚硝态氮、有机氮模拟废水和铵态氮与硝态氮、铵态氮与亚硝态氮混合模拟废水,研究耐冷反硝化细菌Arthrobacter arilaitensis Y-10的异养硝化、好氧反硝化以及同时硝化和反硝化能力, 通过测定Y-10菌株在整个脱氮过程中的D600值,分析细菌生长与生物脱氮之间的联系. 结果表明,耐冷菌株Arthrobacter arilaitensis Y-10具有很强的硝化和反硝化能力,15℃条件下,4 d内分别可将铵态氮由208.43 mg ·L-1降至72.92 mg ·L-1,去除率65.0%; 硝态氮由201.16 mg ·L-1 降至0 mg ·L-1,去除率为100%; 亚硝态氮由194.33 mg ·L-1降至75.43 mg ·L-1,去除率为61.2%. 该菌只在含硝态氮的模拟废水中才会产生亚硝态氮积累; 此外,在混合模拟废水中,以去除铵态氮为主. 总之,Arthrobacter arilaitensis Y-10能在15℃条件下有效进行异养硝化和好氧反硝化作用,在不同无机氮混合模拟废水中对铵态氮的去除率高达80.0%以上. |
| 英文摘要 |
| High concentrations of ammonium, nitrate and nitrite nitrogen were employed to clarify the abilities of heterotrophic nitrification and aerobic denitrification of Arthrobacter arilaitensis strain Y-10. Meanwhile, by means of inoculating the strain suspension into the mixed ammonium and nitrate, ammonium and nitrite nitrogen simulated wastewater, we studied the simultaneous nitrification and denitrification ability of Arthrobacter arilaitensis strain Y-10. In addition, cell optical density was assayed in each nitrogen removal process to analyze the relationship of cell growth and nitrogen removal efficiency. The results showed that the hypothermia denitrification strain Arthrobacter arilaitensis Y-10 exhibited high nitrogen removal efficiency during heterotrophic nitrification and aerobic denitrification. The ammonium, nitrate and nitrite removal rates were 65.0%, 100% and 61.2% respectively when strain Y-10 was cultivated for 4 d at 15℃ with initial ammonium, nitrate and nitrite nitrogen concentrations of 208.43 mg ·L-1, 201.16 mg ·L-1 and 194.33 mg ·L-1 and initial pH of 7.2. Nitrite nitrogen could only be accumulated in the medium containing nitrate nitrogen during heterotrophic nitrification and aerobic denitrification process. Additionally, the ammonium nitrogen was mainly removed in the inorganic nitrogen mixed synthetic wastewater. In short, Arthrobacter arilaitensis Y-10 could conduct nitrification and denitrification effectively under aerobic condition and the ammonium nitrogen removal rate was more than 80.0% in the inorganic nitrogen mixed synthetic wastewater. |
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