| 基于平面波导型荧光免疫传感器的双酚A检测适用性研究 |
| 摘要点击 3242 全文点击 1509 投稿时间：2014-05-17 修订日期：2014-08-11 |
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| 中文关键词 双酚A 环境雌激素 平面波导型荧光免疫传感器 基质效应 免疫分析法 |
| 英文关键词 bisphenol A environmental estrogens planar waveguide fluorescent biosensor matrix effects immunoassay |
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| 中文摘要 |
| 双酚A是一种环境内分泌干扰物,逐渐受到国内外广泛关注. 平面波导型荧光免疫传感器可以对水样中存在的痕量双酚A进行快速高灵敏度检测. 在最优检测条件下测得双酚A标准曲线的检出限为(0.04±0.007)μg·L-1,线性区间为0.16~22.40 μg·L-1,半抑制浓度为(1.67±0.47)μg·L-1. 加入0.5%的EDTA到样品溶液中可以削弱水体硬度的干扰,并在最优条件下测得4种实际水样的加标回收率在88%~111%之间,相对标准偏差小于15%,表明该方法可以运用于实际水样中双酚A的检测. |
| 英文摘要 |
| As a kind of environmental endocrine disruptors, bisphenol A received a wide attention around the world. The planar waveguide fluorescent biosensor can rapidly and sensitively detect traceable bisphenol A in water samples. Under the optimized test conditions, the typical calibration curve for BPA determination by the biosensor showed a detection limit of(0.04±0.007) μg·L-1. The linear response ranged from 0.16 μg·L-1 to 22.40 μg·L-1. The 50% inhibition concentration(IC50) was(1.67±0.47) μg·L-1. By means of adding 0.5% EDTA to sample solutions could weaken the interference of water hardness. Under the optimal conditions, the recovery ratios for four kinds of real water samples were in the range of 88% to 111%, with R. S. D. less than 15%. The results indicate that this method features the potential in the practical detections of bisphenol A in real water samples. |
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