| 长期施用猪粪水稻土抗生素抗性基因污染研究 |
| 摘要点击 5240 全文点击 2670 投稿时间：2014-03-13 修订日期：2014-04-17 |
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| 中文关键词 猪粪 水稻土 抗生素 抗生素抗性基因 高通量定量PCR |
| 英文关键词 manure paddy soil antibiotic antibiotic resistance genes high-throughput qPCR |
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| 中文摘要 |
| 为了全面研究猪粪有机肥对水稻土抗生素抗性及分布格局的影响，选择295对抗生素抗性基因引物，采用高通量荧光定量PCR对水稻土中的抗生素抗性基因污染情况进行了研究. 结果表明，未施用猪粪水稻土检测出66种抗性基因，而施用猪粪水稻土则检测出107种抗生素抗性基因，施用猪粪后水稻土中抗生素抗性基因种类显著增加（P<0.05）. 相对于未施用猪粪水稻土，施用猪粪水稻土有49种抗生素抗性基因丰度显著增加（P<0.05），其中施用猪粪水稻土中喹诺酮类/氯霉素类抗性基因的mexF的丰度相对于未施用猪粪水稻土增加1791倍. 高通量定量结果表明，施用猪粪的水稻土抗生素抗性基因分布格局发生显著变化，高通量定量技术是研究环境抗生素抗性基因的一个非常实用的工具. |
| 英文摘要 |
| To investigate the effect of long-term manure application on the profiles of antibiotic resistance genes (ARGs) in paddy soil, the abundance of ARGs targeted by 295 primers sets were measured by using high-throughput quantitative PCR technique. A total of 107 types of ARGs were detected in long-term manured paddy soil, which is significantly higher than that in control paddy soil (P<0.05). PCA analysis and UPGMA cluster demonstrated that long-term manure application significantly changed the ARGs diversity and abundance in paddy soils. There were 49 types antibiotic resistance genes were enriched significantly in paddy soil applied with manure (P<0.05), especially the mexF, which belongs to quinolones-chloramphenicol resistance genes, increased 1791-fold compared with paddy soil of no manure application. Our results showed that long-term application of manure in paddy soil resulted in the shift of abundance and diversity of ARGs, high-throughput quantitative PCR could be a powerful tool concerning the environmental ARGs dimensions. |
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