| 1株铁基质自养反硝化菌的脱氮特性 |
| 摘要点击 3652 全文点击 2093 投稿时间：2013-08-09 修订日期：2013-10-14 |
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| 中文关键词 铁基质自养反硝化菌 生物脱氮 自养反硝化 硝酸盐 |
| 英文关键词 nitrate-dependent Fe(Ⅱ)-oxidizing bacteria biological nitrogen removal autotrophic denitrification nitrate |
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| 中文摘要 |
| 从武汉市东湖深层底泥中分离得到1株铁基质自养反硝化细菌W5，对其自养反硝化脱氮性能进行了研究.结合生理生化试验和16S rRNA基因序列分析，初步鉴定菌株W5属于微杆菌属（Microbacterium sp.）.对其脱氮能力和影响因素的研究结果表明，W5菌株的最适脱氮培养条件为NO3--N 40mg·L-1，Fe2+ 500mg·L-1，pH 6.8~7.0.在最适脱氮条件下培养一周，硝酸氮去除率可达到87.0%，在整个培养过程中亚硝氮产生量很少，最高不超过0.31mg·L-1.同时未见有氨氮生成，硝酸氮大部分转化成N2.作为电子供体的Fe2+的氧化率达到95.2%. |
| 英文摘要 |
| A nitrate-dependent Fe(Ⅱ)-oxidizing bacterial strain, named W5, was isolated from the sediment of the East Lake in Wuhan. Strain W5 was studied for its characteristics of denitrification and nitrogen removal. According to its physiological and biochemical characteristics and the analysis of its 16S rRNA gene sequence, strain W5 was identified as Microbacterium sp. The optimal denitrification performance can be obtained under conditions of NO3--N 40 mg·L-1, Fe2+ 500 mg·L-1 and pH 6.8-7.0. After one week of cultivation under optimal conditions, nitrate removal percentage reached 87.0%. During the process of the culture, the nitrite nitrogen concentration was no more than 0.31 mg·L-1 and there was no ammonia nitrogen production. It was indicated that the nitrate was mostly converted into N2. The consumption rate of Fe2+ was 95.2%. |
