| 荒漠绿洲土壤抗生素抗性基因分布特征及驱动机制 |
| 摘要点击 1452 全文点击 436 投稿时间：2022-08-12 修订日期：2022-09-19 |
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| 中文关键词 绿洲 土壤 高通量PCR 抗生素抗性基因(ARGs) 土地利用 |
| 英文关键词 oasis soil high throughput PCR antibiotic resistance genes(ARGs) land use |
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| 中文摘要 |
| 荒漠绿洲农田生态系统是干旱区环境下人类活动显著的复合生态系统.土壤微生物抗生素抗性与人类健康和生态平衡关系密切.研究荒漠绿洲环境不同土地利用类型模式下土壤抗生素抗性基因的多样性、分布特征和影响因素，对于评估干旱区土壤环境健康风险，促进绿洲农业生态的发展具有重要意义.采用高通量测序和高通量定量PCR技术对荒漠绿洲土壤微生物的群落结构和抗生素抗性基因多样性开展了研究，旨在探究干旱区土壤抗性基因的分布特征及其驱动机制.结果表明，从沙漠边缘到绿洲，荒漠沙生植物土壤、棉花地土壤、玉米地土壤、芦苇地土壤和湖泊沉积物中抗生素抗性的种类和丰度显著增加，与土地利用变化关系密切，农田土壤是抗性基因的重要存储库；荒漠绿洲土壤微生物群落与抗生素抗性基因显著相关，硫杆菌属（Thiobacillus）、沙漠细菌属（Pontibacter）、诺卡氏菌属（Nocardioides）、耐盐微杆菌属（Salinimicrobium）、土壤红杆菌属（Solirubrobacter）和链霉菌属（Streptomyces）等是各类抗性基因重要的潜在携带者；干旱区土壤中重（类）金属元素和可移动基因元件，与微生物群落共同塑造了抗生素抗性基因的分布格局，这些影响因素单独或者共同的作用，对抗性基因变化解释率总共达到了70%，驱动了荒漠绿洲土壤抗性基因赋存与演化. |
| 英文摘要 |
| The oasis agro-ecosystem is a complex ecosystem with intensive human activities in arid areas. Microbial antibiotic resistance is posing threats to human health and ecological balance. It is of great importance to investigate the diversity, distribution profiles, and driving factors of soil antibiotic resistance genes under different land use patterns in a desert-oasis continuum, especially for assessing soil environmental and human health risks in arid regions. In this study, high throughput sequencing combined with high throughput quantitative PCR were used to investigate the microbial community structure and patterns of antibiotic resistance genes in a desert-oasis continuum, aiming to explore the distribution characteristics and driving mechanisms of soil resistance genes. The results showed that the diversity and abundance of antibiotic resistance increased significantly from the edge of desert to the central oasis, along with Dest, Cotn, Maiz, Reed, and Sedt, consecutively, implying that farmland soil was an important reservoir of resistance genes, which was closely related to land use and land cover change. Soil microbial communities were significantly correlated with antibiotic resistance genes. Thiobacillus, Pontibacter, Nocardioides, Salinimicrobium, Solirubrobacter, and Streptomyces were important potential hosts of various resistance genes. The patterns of antibiotic resistance genes were shaped by heavy metal elements, MGEs, and microbial communities in arid soil, which accumulatively accounted for 70% of the variations in resistance genes alone or together and therefore drove the occurrence, enrichment, and evolution of resistance genes in agricultural soil of the desert-oasis continuum. |
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