| 微塑料诱导下污泥造粒潜能变化及微生物富集特征 |
| 摘要点击 1437 全文点击 431 投稿时间：2022-08-21 修订日期：2022-10-13 |
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| 中文关键词 微塑料(MPs) 污泥 造粒 系统潜能 微生物富集 |
| 英文关键词 microplastics (MPs) sludge granulation system performance microbial enrichment |
| 作者 | 单位 | E-mail | | 谢晴帆 | 浙江万里学院生物与环境学院, 宁波 315100 | 771053440@qq.com | | 俞楠 | 浙江万里学院生物与环境学院, 宁波 315100 | | | 张妮 | 浙江万里学院生物与环境学院, 宁波 315100
湖南大学环境科学与工程学院, 长沙 410082 | | | 谢周云 | 浙江万里学院生物与环境学院, 宁波 315100 | | | 单珂欣 | 浙江万里学院生物与环境学院, 宁波 315100 | | | 吴亦馨 | 浙江万里学院生物与环境学院, 宁波 315100 | | | 唐力 | 浙江万里学院生物与环境学院, 宁波 315100 | | | 夏静芬 | 浙江万里学院生物与环境学院, 宁波 315100 | | | 杨国靖 | 浙江万里学院生物与环境学院, 宁波 315100
湖南大学环境科学与工程学院, 长沙 410082 | guojing_yang@163.com |
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| 中文摘要 |
| 微塑料（MPs）是污水处理厂中普遍检出的新兴污染物之一，目前研究主要集中于传统污水处理系统的污染水平及分布特征，但有关微塑料暴露对污泥颗粒化过程的研究鲜见报道.为探究微塑料对污泥颗粒化的诱导影响，选用环境中广泛检出的聚对苯二甲酸乙二醇酯微塑料（PET-MPs）作为研究对象，通过微塑料暴露试验研究PET-MPs对污泥造粒过程中系统潜能、胞外聚合物（EPS）组成和菌群富集特征的影响.结果表明，PET-MPs暴露显著加快污泥颗粒化进程，同时以蛋白质（PN）为主导的EPS含量上升会增强污泥表面疏水性，造粒速度和EPS分泌量与暴露粒径成正比，微塑料和EPS协同促进颗粒污泥的形成.然而微塑料持续暴露会导致系统除污性能恶化，250 μm PET-MPs暴露下亚硝酸盐氮积累的负面影响最严重，浓度高达（5.08±0.24） mg ·L-1.高通量测序结果进一步表明，变形菌门（Proteobacteria）和拟杆菌门（Bacteroidota）是促进颗粒污泥形成的主要优势门；红环菌科（Rhodocyclaceae）、鞘氨醇杆菌科（Sphingomonadaceae）、黄杆菌科（Flavobacteriaceae）和红细菌科（Rhodanobacteraceae）通过增加分泌EPS促进污泥絮体凝聚；但丛毛单胞菌科（Comamonadaceae）和几丁质菌科（Chitinophagaceae）的相对丰度降低会削弱氨氧化和亚硝酸盐氧化能力；红杆菌科（Rhodobacteraceae）、生丝单胞菌科（Hyphomonadaceae）和黄单胞菌科（Xanthomonadaceae）丰度降低则抑制硝态氮的去除. |
| 英文摘要 |
| Microplastics (MPs), as a new type of pollutant, are widely detected in sewage treatment plants. Currently, research on MPs in traditional sewage treatment systems has mainly been focused on the pollution level and distribution characteristics, with a lack of studying the impact of MPs on the sludge granulation. In order to explore the effect of MPs on the granulation process, a microplastic exposure test was conducted by adding polyethylene terephthalate microplastics (PET-MPs), which are widespread in the environment. The operating performance of the system, extracellular polymeric substance (EPS) composition, and flora enrichment were analyzed on the sludge granulation. The results showed that the exposure of PET-MPs significantly accelerated the sludge granulation process, whereas the increase in EPS content dominated by PN enhanced the sludge surface hydrophobicity; the granulation rate and EPS secretion were proportional to the exposed particle size. Microplastics and EPS secretions synergistically promoted the formation of granular sludge. However, continuous microplastic exposure led to deterioration of the system decontamination performance and inhibited the degradation process of pollutants, with the most negative effect of nitrite nitrogen accumulation under 250 μm PET-MPs exposure, as high as (5.08±0.24) mg·L-1. The high-throughput sequencing revealed that the microbial community diversity fell in the experimental group. The dominant bacteria at the phylum level were Proteobacteria and Bacteroidota on the sludge granulation. Rhodocyclaceae, Sphingomonadaceae, Flavobacteriaceae, and Rhodanobacteraceae promoted flocculation by increasing EPS secretion. The decrease in Comamonadaceae and Chitinophagaceae weakened the ammonia and nitrite oxidation capacity of the system, whereas the decrease in Rhodobacteraceae, Hyphomonadaceae, and Xanthomonadaceae inhibited the removal of nitrate nitrogen. |
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