| 不同供水管材生物膜抗生素抗性基因分布特征 |
| 摘要点击 1494 全文点击 542 投稿时间：2022-04-17 修订日期：2022-06-11 |
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| 中文关键词 供水管网 不锈钢管 塑料管 生物膜 抗生素抗性基因(ARGs) |
| 英文关键词 water supply network stainless steel pipe plastic pipe biofilm antibiotic resistance genes(ARGs) |
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| 中文摘要 |
| 为了研究不同材质供水管道内壁生物膜中抗生素抗性基因分布特征和水平转移潜力，利用宏基因组测序对304、316 L不锈钢管和PPR、PE塑料管内壁生物膜进行了分析.结果表明，4种管材内壁生物膜中共检出17类146种抗生素抗性基因.多药类抗性基因在不同管材生物膜中的丰度最高且差别较大，是不同管材生物膜抗生素抗性基因差异的主要原因.不同管材生物膜中可移动遗传元件的总丰度水平较高，但整体上和抗生素抗性基因共存的比例较低，其中整合酶基因表现出和抗生素抗性基因更加密切的关系，可能在抗生素抗性基因的水平转移上发挥重要作用.不同的抗性基因拥有不同种类的潜在宿主，水杆菌属（Aquabacterium）、慢生根瘤菌属（Bradyrhizobium）等高丰度宿主是多药类抗性基因大量存在的基础.不同管材管壁生物膜中抗生素抗性的首要影响因素是细菌群落组成，其次是可移动遗传元件介导的基因水平转移；物种组成和可移动遗传元件共同塑造了抗性基因的分布特征. |
| 英文摘要 |
| To study the distribution characteristics and horizontal transfer potential of antibiotic resistance genes in biofilms of water supply pipes made of different materials, the biofilms of 304 and 316 L stainless steel pipes and PPR and PE plastic pipes were analyzed using metagenomic sequencing. The results showed that a total of 146 antibiotic resistance genes belonging to 17 classes were detected in the biofilms. Multidrug resistance genes had the highest abundance and varied a lot in different biofilms, which was the main reason for the differences in antibiotic resistance genes in different biofilms. The total abundance of mobile genetic elements was high in different biofilms, but overall coexistence with antibiotic resistance genes was low, with integrase genes showing a closer relationship with antibiotic resistance genes and possibly playing an important role in the horizontal transfer of antibiotic resistance genes. Different antibiotic resistance genes possessed different genera of potential hosts; highly abundant hosts such as Aquabacterium and Bradyrhizobium were the basis for the presence of a high abundance of multidrug resistance genes. Microbial attachment in different pipe walls was selective, and the primary influence of antibiotic resistance in biofilms was bacterial community composition, followed by the horizontal transfer of genes mediated by mobile genetic elements, community composition, and mobile genetic elements together shaping the distribution characteristics of antibiotic resistance genes. |
