| 模拟及实际根系分泌物对芘污染土壤微生物群落的影响 |
| 摘要点击 2381 全文点击 731 投稿时间：2021-03-24 修订日期：2021-07-14 |
| 查看HTML全文
查看全文 查看/发表评论 下载PDF阅读器 |
| 中文关键词 根系分泌物 芘 土壤 微生物群落 高通量测序技术 |
| 英文关键词 root exudates pyrene soil microbial community high-throughput sequencing method |
| 作者 | 单位 | E-mail | | 韩博远 | 齐鲁工业大学(山东省科学院), 山东省科学院生态研究所, 山东省应用微生物重点实验室, 济南 250103 | 2453605137@qq.com | | 张闻 | 齐鲁工业大学(山东省科学院), 山东省科学院生态研究所, 山东省应用微生物重点实验室, 济南 250103 | zw-sunshine@163.com | | 胡芳雨 | 齐鲁工业大学(山东省科学院), 山东省科学院生态研究所, 山东省应用微生物重点实验室, 济南 250103 | | | 赵庆庆 | 齐鲁工业大学(山东省科学院), 山东省科学院生态研究所, 山东省应用微生物重点实验室, 济南 250103 | | | 卢媛 | 南开大学环境科学与工程学院, 天津 300350 | | | 孔学 | 齐鲁工业大学(山东省科学院), 山东省科学院生态研究所, 山东省应用微生物重点实验室, 济南 250103 | | | 吕俊岗 | 最高人民检察院检察技术信息研究中心, 北京 100144 | |
|
| 中文摘要 |
| 为了解根系分泌物对芘污染土壤中微生物群落的影响，应用Illumina NovaSeq高通量测序技术，研究了模拟根系分泌物和实际黑麦草根系分泌物添加至芘污染土壤后，细菌群落组成、α和β多样性以及基因功能的变化.结果表明，根系分泌物未明显改变芘污染土壤细菌群落的组成，土壤细菌群落中主要优势菌门为变形菌门、放线菌门、厚壁菌门和拟杆菌门等；主要优势菌属为鞘氨醇单胞菌属、乳酸杆菌属和芽孢杆菌属等.根系分泌物的添加导致优势物种相对丰度发生了不同程度的变化，并产生了差异物种.隶属变形菌门的毛螺旋菌属和隶属厚壁菌门的瘤胃梭菌属分别为模拟根系分泌物组和黑麦草实际根系分泌物组的标志性差异物种.2个根系分泌物组相较于对照组的共同差异物种均为多环芳烃（polycyclic aromatic hydrocarbons，PAHs）降解优势菌，根系分泌物的添加选择性地促进了PAHs降解菌的生长.根系分泌物对芘污染土壤细菌群落的丰富度和多样性影响不大，但对群落结构的影响较为显著，主要归因于低丰度物种的较大变化.模拟根系分泌物和实际根系分泌物处理组之间的群落结构较为相近.根系分泌物使土壤中的芘含量分别降低了14.0%和8.7%，归因于其对PAHs降解菌的生长促进以及部分功能基因丰度的显著增加.结果可为研究植物根际修复PAHs污染土壤的作用机制提供数据支撑. |
| 英文摘要 |
| The high-throughput Illumina NovaSeq sequencing method was adopted to study the effect of artificial root exudates and Lolium perenne L. root exudates on the community structure, α and β diversity, and gene function of the bacterial communities in pyrene-contaminated soils to understand the impact of root exudates on microbial communities. The results showed that root exudates did not significantly change the composition of pyrene-contaminated soil bacterial communities. The main dominant bacterial phyla were Proteobacteria, Actinobacteria, Firmicutes, Bacteroidetes, etc. The main dominant bacterial genera were Sphingomonas, Lactobacillus, Bacillus, etc. Root exudates changed the relative abundance of dominant species to a different extent and resulted in discriminating bacteria. The genus Lachnospiraceae belonging to Proteobacteria and Ruminiclostridium belonging to Firmicutes were the biomarkers in the artificial root exudates group and the actual root exudate group, respectively. The common discriminating bacteria in both root exudate groups compared to those in the control group were polycyclic aromatic hydrocarbon (PAHs)-degrading bacteria. Root exudates selectively promoted the growth of PAHs-degrading bacteria. Root exudates had little effect on the richness and diversity of the bacterial communities in pyrene-contaminated soil. However, they significantly influenced the soil bacterial community structure, which resulted from significant changes in low-abundance species. The bacterial community structures of the two root exudate groups were similar. Root exudates decreased pyrene concentration in the soil by 14.0% (artificial root exudates) and 8.7% (actual root exudates). The promotion of pyrene degradation affected by root exudates was due to the growth promotion of PAHs-degrading bacteria and the significant increase in the abundance of some functional genes. This research can supply data for the exploration of a rhizoremediation mechanism in PAHs-contaminated soils. |
|
|
|
