| 生物质炭影响下土壤呼吸温度敏感性及细菌群落结构的变化 |
| 摘要点击 1581 全文点击 830 投稿时间：2020-04-20 修订日期：2020-05-16 |
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| 中文关键词 生物质炭(BC) 土壤呼吸 温度敏感性 土壤有机碳组分 细菌群落结构 |
| 英文关键词 biochar(BC) soil respiration temperature sensitivity soil organic carbon fractions bacterial community structure |
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| 中文摘要 |
| 为探究生物质炭对土壤呼吸及微生物群落结构的影响，在不同生物质炭用量及不同温度条件下进行室内土壤培养试验，分析土壤CO2释放规律、不同有机碳组分的变化以及土壤细菌群落结构特征.结果表明：①生物质炭处理对各温度条件下的土壤呼吸均表现为前期激发和后期抑制的效应，且在培养14 d之后，生物质炭处理显著降低了土壤呼吸温度敏感性Q10值.②就土壤有机碳而言，未添加生物质炭的CK处理土壤惰性有机碳含量的下降幅度随温度升高而增大，对温度变化较敏感，但生物质炭的输入显著降低了土壤惰性有机碳的温度敏感性，使各温度条件下土壤惰性有机碳的降幅收窄，且降幅并未随温度的升高而增大.③16S rDNA高通量测序结果显示，至培养末期，CK处理土壤中马赛菌属（Massilia）的相对丰度随温度升高而降低，且生物质炭的输入显著提高了马赛菌属的温度敏感性，使其相对丰度随温度升高降幅增大.与马赛菌属相反，CK处理土壤中嗜盐囊菌属（Haliangium）的相对丰度随温度的升高显著增加，生物质炭的输入降低了其温度敏感性，使温度梯度间相对丰度差异不显著.本研究表明，生物质炭的输入可显著降低土壤呼吸温度敏感性，这与土壤惰性有机碳温度敏感性的降低及马赛菌属和嗜盐囊菌属相对丰度的改变有关. |
| 英文摘要 |
| To explore the effects of biochar on the temperature sensitivity of soil respiration and microbial community structure, the soil was subjected to an indoor culture test under two major treatments which were control (CK) and the addition of 3% (mass ratio) biochar (BC). Each major treatment contained four temperature conditions. We analyzed the changes in soil CO2 emission, the content of different soil organic carbon, and the characteristics of the soil bacterial community. The results showed that:①The input of biochar to soil respiration under various temperature conditions was promoted in the early stage and inhibited in the later stage, and after 14 days of cultivation, the input of biochar significantly reduced the temperature sensitivity Q10 values of soil respiration. ② For soil organic carbon fractions, the decline rate of the content of soil inert organic carbon under the CK treatment increased with increasing temperature, and was sensitive to the changes in temperature. However, the input of biochar significantly reduced the temperature sensitivity of the soil inert organic carbon, and narrowed its decline rate under each temperature condition. The decline rate did not increase with increasing temperature. ③The 16S rDNA high-throughput sequencing results showed that at the end of the cultivation, the relative abundance of Massilia in the soil of the CK treatment decreased with increasing temperature, and the input of biochar significantly increased its temperature sensitivity, making the decline rate increase. In contrast to Massilia, the relative abundance of Haliangium in soil of the CK treatment increased significantly with increasing temperature, and the input of biochar significantly reduced its temperature sensitivity, rendering the difference of relative abundance between each temperature condition not significant. This study shows that the input of biochar can significantly reduce the temperature sensitivity of soil respiration, which is related to decreased temperature sensitivity of soil inert organic carbon, and the change in the relative abundance of Massilia and Haliangium after the input of biochar. |
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