| 连续流SNAD工艺处理猪场沼液启动过程中微生物种群演变及脱氮性能 |
| 摘要点击 1189 全文点击 494 投稿时间：2019-10-07 修订日期：2019-12-17 |
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| 中文关键词 SNAD工艺 猪场沼液 高通量测序技术 定量PCR 微生物种群 |
| 英文关键词 simultaneous partial nitrification, ANAMMOX, and denitrification (SNAD) anaerobic digester liquor of swine wastewater (ADLSW) high-throughput sequencing quantitative PCR bacterial community shifts |
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| 中文摘要 |
| 为了实现合建式连续流同步部分亚硝化、厌氧氨氧化和反硝化SNAD（simultaneous partial nitrification，ANAMMOX，and denitrification）工艺处理实际猪场沼液，保持温度为（30±1）℃，控制溶解氧（DO）为（0.4±0.1）mg·L-1，首先通过逐步提高模拟进水氨氮浓度来实现SNAD工艺的启动，然后实现SNAD工艺处理实际猪场沼液的稳定运行.同时，采用高通量测序和实时定量PCR（qPCR）技术对反应器启动前后及沼液替换成功时关键生物种群进行分析.结果表明，150 d左右可实现SNAD工艺的启动，298 d完成实际沼液的替换，其出水（NO3--N+NO2--N）/ΔNH4+-N小于0.11，对NH4+-N和TN的平均去除率为63.26%和55.71%.高通量测序结果表明，绿弯菌门（Chloroflexi，相对丰度50.78%）、变形菌门（Proteobacteria，13.34%）、浮霉菌门（Planctomycetes，9.26%）是沼液替换成功时污泥中的优势菌门；主要优势脱氮菌属Nitrosomonas的相对丰度由启动前1.55%增加到1.98%；两类具有厌氧氨氧化（ANAMMOX）功能菌Candidatus_Brocadia和Candidatus_Kuenenia的相对丰度分别从启动前0.01%和未检出（<0.01%）增加到4.66%和4.18%；Denitratisoma作为主要的反硝化菌，丰度由启动前未检出（<0.01%）增加到2.06%.qPCR结果表明，与接种污泥相比，沼液替换成功后AOB、ANAMMOX菌和反硝化菌的含量均有明显增加.将SNAD工艺用于实际猪场沼液处理，可实现高效稳定脱氮，节约后续处理成本. |
| 英文摘要 |
| To realize a simultaneous partial nitrification, ANAMMOX (anaerobic ammonium oxidation), and denitrification (SNAD) process treating anaerobic digester liquor of swine wastewater (ADLSW) in a continuous-flow biofilm reactor (CFBR), we first gradually increased the influent ammonium (NH4+-N) concentration, and then enhanced the ADLSW ratio in the influent during operation; dissolved oxygen (DO) was controlled at (0.4±0.1) mg·L-1 by adjusting the air flow rate, and the temperature was kept at (30±1)℃. Meanwhile, high-throughput sequencing and quantitative PCR (polymerase chain reaction) techniques were used to analyze the bacterial community shifts and the amount of dominant nitrogen removal bacteria. The results demonstrated that a successful start-up of the SNAD process was accomplished in 150 d, and replacement of the actual biogas slurry was completed in 298 d. The effluent (NO3--N+NO2--N)/ΔNH4+-N value was less than 0.11, and the average removal rates of NH4+-N and TN (total nitrogen) increased to 63.26% and 55.71%, respectively. Moreover, high-throughput sequencing results demonstrated that the dominant microbial populations at phylum level were Chloroflexi (with a relative abundance of 50.78%), Proteobacteria (13.34%), and Planctomycetes (9.26%). The relative abundance of Nitrosomonas increased from 1.55% to 1.98%. In addition, the relative abundance of Candidatus_Brocadia and Candidatus_Kuenenia increased from 0.01% and (<0.01%) to 4.66% and 4.18%, respectively, and the relative abundance of Denitratisoma increased from (<0.01%) to 2.06%. Meanwhile, qPCR analysis showed that the amounts of ammonia-oxidizing bacteria, ANAMMOX, and denitrifying bacteria increased significantly compared with the inoculated sludge. An efficient and stable nitrogen removal rate can be achieved, and the follow-up processing cost can be reduced, by application of the SNAD treatment process for ADLSW. |
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