| 潜流人工湿地基质结构与微生物群落特征的相关性 |
| 摘要点击 2530 全文点击 1001 投稿时间：2017-01-07 修订日期：2017-04-09 |
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| 中文关键词 人工湿地 基质结构 微生物群落 高通量 主成分分析 |
| 英文关键词 constructed wetlands substrate structure microbial community high throughput PCA |
| 作者 | 单位 | E-mail | | 李振灵 | 桂林理工大学广西矿冶与环境科学实验中心, 桂林 541004 | LiZhenLing1992@163.com | | 丁彦礼 | 桂林理工大学广西矿冶与环境科学实验中心, 桂林 541004 | dyl@glut.edu.cn | | 白少元 | 桂林理工大学广西矿冶与环境科学实验中心, 桂林 541004
广西环境污染控制理论与技术重点实验室, 桂林 541004 | | | 李雪芬 | 桂林理工大学广西矿冶与环境科学实验中心, 桂林 541004
广西环境污染控制理论与技术重点实验室, 桂林 541004 | | | 游少鸿 | 桂林理工大学广西矿冶与环境科学实验中心, 桂林 541004
广西环境污染控制理论与技术重点实验室, 桂林 541004 | | | 解庆林 | 桂林理工大学广西矿冶与环境科学实验中心, 桂林 541004
广西环境污染控制理论与技术重点实验室, 桂林 541004 | |
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| 中文摘要 |
| 为查明造成不同基质结构人工湿地净化性能差异的微生物因素，利用高通量测序技术，研究水平潜流人工湿地基质结构与微生物群落结构的相关性.结果表明，3个对比人工湿地系统中，从床体表层到底层渗透系数逐渐升高的六层结构人工湿地（CW6）净化性能最高，其出水中COD、TN、NO3--N及NH4+-N平均浓度分别为39、11、0.35及4mg·L-1，其后为三层结构人工湿地（CW3），而单层结构人工湿地（CW1）的净化效果最差，平均出水浓度分别为95、21、0.60及12mg·L-1.高通量测序结果显示，多层结构人工湿地中微生物OTUs略低于单层结构湿地，但其优势菌门变形菌门（Proteobacteria）和其属水平上的硝化与反硝化细菌的相对丰度却显著高于单层湿地.PCA和丰度热图分析结果显示，CW3和CW6床体中变形菌门（Proteobacteria）属水平上的微生物存在显著的空间分布差异，从而有利于污染物的分区降解，而CW1各样点间的微生物群落结构无显著性差异. |
| 英文摘要 |
| To identify the microbial factors that cause the differences in the purification performance of constructed wetlands with different substrate structures, the relationship between the substrate structure and the microbial community composition in horizontal subsurface flow constructed wetlands (HSSFCWs) was studied by high throughput sequencing. The results revealed that the purification performance of a six-layer constructed wetland (CW6), of which the permeability coefficient gradually increased from the surface layer to the bottom layer, was the highest among the three constructed wetland systems. The average concentrations of COD, TN, NO3--N, and NH4+-N in the effluent were 39, 11, 0.35, and 4 mg·L-1, respectively. The monolayer structure constructed wetland (CW1) had the worst purifying efficiency, with average effluent concentrations of 95, 21, 0.60 and 12 mg·L-1 for COD, TN, NO3--N, and NH4+-N, respectively. The results of the high-throughput sequencing showed that the number of microbial OTUs in multilayer structure wetlands was slightly lower than that in the monolayer structure wetland, but the relative abundance of the dominant phylum Proteobacteria and the nitrifying and denitrifying bacteria in the genus was significantly higher than the monolayer structure wetland. The results of PCA and heatmap indicated that there were significant differences in the spatial distribution of microbes in the genus of Proteobacteria in CW3 and CW6, which facilitated the degradation of pollutants. No significant differences were found in the community structure of CW1. |
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