| 秦岭红桦林土壤细菌群落剖面分布特征及其影响因素 |
| 摘要点击 1761 全文点击 624 投稿时间：2016-11-01 修订日期：2017-01-19 |
| 查看HTML全文
查看全文 查看/发表评论 下载PDF阅读器 |
| 中文关键词 红桦林 高通量测序 土壤细菌 土壤层 土壤因素 |
| 英文关键词 Betula albosinensis Burkill forest Illumina MiSeq high-throughput sequencing soil bacteria soil depth soil factors |
| 作者 | 单位 | E-mail | | 杜璨 | 西北农林科技大学资源环境学院, 农业部西北植物营养与农业环境重点实验室, 杨凌 712100 | ducan616@163.com | | 许晨阳 | 西北农林科技大学资源环境学院, 农业部西北植物营养与农业环境重点实验室, 杨凌 712100 | | | 王强 | 西北农林科技大学资源环境学院, 农业部西北植物营养与农业环境重点实验室, 杨凌 712100 | | | 张帆 | 陕西省宝鸡市辛家山林业局, 宝鸡 721700 | | | 马武功 | 陕西省宝鸡市辛家山林业局, 宝鸡 721700 | | | 和文祥 | 西北农林科技大学资源环境学院, 农业部西北植物营养与农业环境重点实验室, 杨凌 712100 | | | 侯琳 | 西北农林科技大学林学院, 杨凌 712100 | | | 耿增超 | 西北农林科技大学资源环境学院, 农业部西北植物营养与农业环境重点实验室, 杨凌 712100 | gengzengchao@126.com |
|
| 中文摘要 |
| 研究秦岭辛家山林区红桦林细菌群落在土壤剖面上的分布状况，对评估土壤细菌在森林生态系统土壤肥力调节、碳氮循环等作用至关重要.采用Illumina MiSeq高通量测序技术对土壤细菌16S rDNA V3~V4可变区进行测序，结合相关生物信息学分析，初步探讨了红桦林0~10、10~20、20~40和40~60 cm这4个土壤层细菌群落丰富度、多样性指数和细菌群落组成及丰度变化.结果表明，在红桦林土壤剖面上，OTUs、Chao1指数、Shannon指数均在0~10 cm处达到最大值，分别为1688、2314、8.66，土层间差异不显著.4个土壤层的优势菌门均为酸杆菌门（Acidobacteria）、变形菌门（Proteobacteria），主要的优势菌属为Gp4、Gp6和Gp16.优势菌门的相对丰度在土层间并不相同，0~10 cm土壤层具有较高的变形菌门（Proteobacteria），其相对丰度为23.62%，而40~60 cm具有较高的酸杆菌门（Acidobacteria），相对丰度为62.88%.酸杆菌门（Acidobacteria）与全氮、土壤有机碳、C/N、可溶性有机碳显著相关，变形菌门（Proteobacteria）与土壤含水量、土壤有机碳、可溶性有机碳显著相关.经RDA分析证明，影响秦岭红桦林土壤剖面细菌群落分布的主要土壤因素是可溶性有机碳.这些研究结果表明在秦岭红桦林土壤4个土层均有较高的细菌多样性，为进一步认识森林土壤细菌多样性奠定了理论基础，在研究森林生态系统土壤剖面养分循环过程时应予以考虑. |
| 英文摘要 |
| In this study, vertical changes in bacterial α-diversity and community composition were investigated at four soil depths(0-10, 10-20, 20-40 and 40-60 cm) in Betula albosinensis Burkill forest of Qinling Mountains by sequencing of the 16S rDNA regions using Illumina MiSeq high-throughput technology. The results showed that the decreases of OTUs, Chao1 and Shannon were numerical but not significant, and the highest values of 1688, 2314 and 8.66 were obtained in 0-10 cm, respectively. At the phylum level, Acidobacteria and Proteobacteria were the most dominant bacteria in four soil layers. At the genus level, Gp4, Gp6 and Gp16 were the most dominant bacteria. The relative abundance of Acidobacteria in 40-60 cm soil depth(62.88%) was higher than those in other soil depths. Proteobacteria in 0-10 cm(23.62%) was more abundant than that in 40-60 cm. The relative abundance of Acidobacteria was significantly correlated with the total N, soil organic carbon, C/N, and soil dissolved organic carbon. Soil water content, soil organic matter and soil dissolved organic carbon were the key factors affecting soil Proteobacteria. RDA sequencing results showed that soil dissolved organic carbon was the key factor contributing to the bacteria community abundance. The results demonstrated that there are plenty of bacterial distribution in all four soil layers, which provides a fundamental basis for vertical soil bacterial community diversity, and possesses very important research value in biogeochemical cycling. |
|
|
|
