| 净水工艺对饮用水中微生物多重耐药性的影响与分析 |
| 摘要点击 1677 全文点击 721 投稿时间：2016-04-11 修订日期：2016-06-01 |
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| 中文关键词 饮用水 净水工艺 耐药细菌 多重耐药 活性炭 |
| 英文关键词 drinking water water treatment process antibiotic resistant bacteria (ARB) multidrug resistance biological activated carbon |
| 作者 | 单位 | E-mail | | 马晓琳 | 上海交通大学生命科学技术学院, 微生物代谢国家重点实验室, 上海 200240 | xlm0510@sjtu.edu.cn | | 陆洋 | 上海交通大学生命科学技术学院, 微生物代谢国家重点实验室, 上海 200240 | | | 王钰 | 上海交通大学生命科学技术学院, 微生物代谢国家重点实验室, 上海 200240 | | | 侯誉 | 上海交通大学生命科学技术学院, 微生物代谢国家重点实验室, 上海 200240 | | | 白晓慧 | 上海交通大学生命科学技术学院, 微生物代谢国家重点实验室, 上海 200240 | xhbai@sjtu.edu.cn |
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| 中文摘要 |
| 由于抗生素过量使用已导致我国部分水源受到多种抗生素和耐药基因的污染，抗生素及其耐药基因可能通过饮用水对人体健康造成严重威胁.对上海某水厂供水系统可培养微生物进行了分离鉴定；使用5种抗生素：氨苄青霉素（Amp）、卡那霉素（Kan）、利福平（Rif）、氯霉素（Cm）以及链霉素（Str）对饮用水中可培养微生物多重耐药性及其在供水系统中的变化规律和耐药机制进行了分析.结果表明：所分离可培养微生物主要属于16个属，其中芽孢杆菌属（Bacillus sp.）、噬纤维菌属（Arcicella rosea sp.）、鞘酯菌属（Sphingomonas sp.）在所有工艺单元中都可分离获得；经过生物活性炭滤池和管网输配后3种菌的多重耐药能力均有增强，其中芽孢杆菌属（Bacillus sp.）耐药能力最强.采用实时荧光定量PCR对供水系统中3种整合子及9种转座子进行定量检测，发现经过生物活性炭滤池和管网输配后，可移动基因元件绝对数量增加明显，生物活性炭滤池和输配管网对饮用水中微生物耐药能力具有重要影响. |
| 英文摘要 |
| The overuse of antibiotics has resulted in contamination of antibiotics and genes encoding multidrug resistance in some water sources in China. Antibiotics and the antibiotic resistance genes may cause severe hazards to human health via drinking water. Cultivable bacteria in one of the water supply systems in Shanghai were isolated and identified. The multidrug resistance in drinking water for cultivable bacteria and their change and mechanism in water supply system was analyzed using ampicillin (Amp), kanamycin (Kan), rifampicin (Rif), chloramphenicol (Cm) and streptomycin (Str). The results showed that, the isolated microorganisms mainly belonged to 16 genera. Bacillus sp., Arcicella rosea sp. and Sphingomonas sp. existed in the whole process. The multidrug resistances of these three bacteria were enhanced after flowing carbon filtration and water distribution system. Bacillus sp. showed the strongest antibiotic resistance. Real-time PCR was used to quantitatively evaluate the concentration of three integrons and 9 transposons in the water supply system. The results showed that, after BAC filtration and water distribution system, the absolute concentrations of mobile genetic elements increased obviously, which meant that BAC filtration and water distribution system played an important role in influencing antibiotic resistance in the water treatment process. |
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