| 浙江省瓯江氨氧化古菌和氨氧化细菌分布及多样性特征 |
| 摘要点击 2779 全文点击 1478 投稿时间：2015-04-27 修订日期：2015-07-16 |
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| 中文关键词 氨氧化古菌 氨氧化细菌 感潮河段 沉积物 群落组成 多样性 |
| 英文关键词 ammonium-oxidizing archaea ammonium-oxidizing bacteria tidal river sediment community composition diversity |
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| 中文摘要 |
| 氨氧化古菌(ammonia-oxidizing archaea,AOA)和氨氧化细菌(ammonia-oxidizing bacteria,AOB)在生物地球化学氮循环过程中发挥着重要作用. 河流是关系人类生产和生活的重要生态系统,蕴含大量氮循环功能微生物. 本研究采用变性梯度凝胶电泳(denaturing gradient gel electrophoresis, DGGE)和荧光定量PCR(quantitative PCR, qPCR)技术对沉积物AOA、AOB群落进行结构和丰度分析,在瓯江感潮河段尺度上探究AOA、AOB分布规律及影响AOA、AOB群落结构与丰度的因素. 结果表明, AOA群落结构差异不显著,影响其分布的主要因素为NH4+和TS; AOB群落结构存在显著差异,序列分析比对表明AOB分为Nitrosospira和Nitrosomonas,其中90%序列为Nitrosospira, EC、pH、NH4+、NO3-、TC和TN是影响AOB群落组成的重要环境因素; 总硫(TS)和电导率(EC)分别是影响AOA和AOB多样性的主要因素; AOA丰度显著高于AOB; EC、NH4+-N和NO3--N是影响AOA和AOB丰度的主要环境因素. 研究表明,瓯江感潮河段沉积物中AOA和AOB群落结构和丰度均显著受环境因素影响,AOA在表层沉积物氨氧化过程中可能占主导位置. |
| 英文摘要 |
| Ammonia-oxidizing archaea (AOA) and ammonia-oxidizing bacteria (AOB) play important roles in the biogeochemical nitrogen cycle. Rivers are important ecosystems containing a large number of functional microbes in nitrogen cycle. In this study, denaturing gradient gel electrophoresis (DGGE) and real-time quantitative PCR (qPCR) technology were used to analyze the distribution and diversity of AOA and AOB in sediments from Oujiang. The results showed that the AOA community structure was similar among various sites, while the AOB community structure was significantly different, in which all detected AOB sequences were classified into Nitrosospira and Nitrosomonas, and 90% affiliated to Nitrosospira. The community composition of AOA was influenced by NH4+ and TS, in addition, the AOB composition was affected by NH4+, EC, pH, NO3-, TC and TN. Total sulfur (TS) and electrical conductivity (EC) were the major factors influencing the diversity of AOA and AOB, respectively. AOA abundance was significantly higher than that of AOB. EC, NH4+-N and NO3--N were the main environmental factors affecting the abundance of AOA and AOB. This study indicated that the community composition and diversity of AOA and AOB were significantly influenced by environmental factors, and AOA might be dominant drivers in the ammonia oxidation process in Oujiang surface sediment. |
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